Comparison of Xpert MRSA/SA Nasal and MRSA/SA ELITe MGB Assays for Detection of the mecA Gene with Susceptibility Testing Methods for Determination of Methicillin Resistance in Staphylococcus aureus Isolates
| Autor: | Maurice Biendo, F. Rousseau, Hedi Mammeri, Farida Hamdad, Brigitte Canarelli, Mohamed Belmekki |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Methicillin-Resistant Staphylococcus aureus Microbiology (medical) Penicillin binding proteins Adolescent Microbial Sensitivity Tests Real-Time Polymerase Chain Reaction medicine.disease_cause Staphylococcal infections Benzylpenicillin Microbiology Young Adult Bacterial Proteins medicine Humans Penicillin-Binding Proteins Cefoxitin Child Aged Aged 80 and over business.industry SCCmec Infant Bacteriology Middle Aged Staphylococcal Infections biochemical phenomena metabolism and nutrition bacterial infections and mycoses medicine.disease Methicillin-resistant Staphylococcus aureus Molecular Diagnostic Techniques Staphylococcus aureus Child Preschool Female business Staphylococcus medicine.drug |
| Zdroj: | Journal of Clinical Microbiology. 51:3183-3191 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.00860-13 |
| Popis: | In a series of 82 Staphylococcus strains isolated from culture, 100% were identified as Staphylococcus aureus by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS); 99.9% (77/82) of them were resistant to benzylpenicillin, oxacillin, and cefoxitin, and 6.1% (5/82) were susceptible to methicillin. Xpert MRSA/SA assay results were concordant with the phenotypic results in 76.8% (63/82) of cases and discordant in 23.2% (19/82) of cases. The MRSA/SA ELITe MGB kit results were concordant with phenotypic results in 100% of the cases. When comparing the Xpert MRSA/SA assay results with the MRSA/SA ELITe MGB kit results, 78% (64/82) of the cases were concordant, while 22% (18/82) of the cases were discordant. No statistically significant differences were observed between the two techniques. The PCR protocol that was used to validate the results of these two methods gave the following results: 49 were conventional methicillin-resistant S. aureus (MRSA) isolates ( mecA positive and mecA LGA251 negative), and 25 were phenotypic MRSA isolates ( mecA negative and mecA LGA251 positive). |
| Databáze: | OpenAIRE |
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