Salmonella enterica serovar typhi: molecular analysis of strains with decreased susceptibility and resistant to ciprofloxacin in india from 2001-2003
| Autor: | Pushpa Aggarwal, Deepthi Nair, Vanessa Mathys, Malini R. Capoor, Marie Dehem, P. Bifani |
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| Jazyk: | angličtina |
| Předmět: |
DNA
Bacterial Microbiology (medical) Serotype lcsh:QR1-502 India Microbial Sensitivity Tests Biology Polymerase Chain Reaction lcsh:Microbiology Agar dilution Microbiology lcsh:Infectious and parasitic diseases resistance Nalidixic Acid Minimum inhibitory concentration Anti-Infective Agents ciprofloxacin Drug Resistance Bacterial medicine Humans lcsh:RC109-216 S.Typhi Salmonella enterica Salmonella typhi Antimicrobial biology.organism_classification Virology Molecular analysis Ciprofloxacin Infectious Diseases Salmonella enterica serovar Typhi Mutation medicine.drug |
| Zdroj: | Brazilian Journal of Infectious Diseases, Vol 11, Iss 4, Pp 423-425 Brazilian Journal of Infectious Diseases v.11 n.4 2007 Brazilian Journal of Infectious Diseases Brazilian Society of Infectious Diseases (BSID) instacron:BSID Brazilian Journal of Infectious Diseases, Volume: 11, Issue: 4, Pages: 423-425, Published: AUG 2007 |
| ISSN: | 1678-4391 |
| Popis: | Chromosomally-mediated reduced susceptibility to ciprofloxacin narrows the therapeutic options in enteric fever. We made a molecular comparison of clinical isolates of fluoroquinolone-resistant strains of Salmonella enterica serotype Typhi from January 2001 to May 2003; 178 isolates were subjected to antimicrobial susceptibility testing by the Kirby-Bauer method of disk diffusion, and agar dilution was used to determine the minimum inhibitory concentration (MIC) to ciprofloxacin. Nalidixic-acid resistant strains (NARST) were observed in 51% of the isolates, of which 98.9% had decreased susceptibility (MICor =0.125-1 microg/mL) to ciprofloxacin. A single strain (4 microg/mL) was resistant to ciprofloxacin and double mutations were found in the gyrA gene (76 Asp-Asn, 44 leu-Ileu). Among seven NARST strains with reduced susceptibility, a single mutation was found in five strains, one of which had 76 Asp-Asn and two each had mutations at 87 Asp-Asn and 72 Phe-Tyr, respectively); no mutations could be detected in two isolates. Routine antimicrobial surveillance, coupled with molecular analysis of fluoroquinolone resistance, is crucial for revision of enteric fever therapeutics. |
| Databáze: | OpenAIRE |
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