The effect of isorhamnetin glycosides extracted from Opuntia ficus-indica in a mouse model of diet induced obesity
| Autor: | Claudia Marquez-Mota, Ivan Torre-Villalvazo, Rocio Alejandra Chavez-Santoscoy, Marilena Antunes-Ricardo, Guillermo Ordaz, Sergio O. Serna-Saldívar, Janet A. Gutiérrez-Uribe, Ana María Leal-Díaz, Lilia G. Noriega, César Rodríguez-Rodríguez, Armando R. Tovar, Nimbe Torres |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Hyperlipidemias Type 2 diabetes Diet High-Fat Tissue Culture Techniques chemistry.chemical_compound Islets of Langerhans Random Allocation Adipocyte Internal medicine Insulin Secretion medicine Animals Insulin Glycosides Obesity Rats Wistar Beta oxidation Isorhamnetin Glucose Transporter Type 2 biology Plant Stems Cholesterol Plant Extracts Opuntia General Medicine medicine.disease IRS1 Mice Inbred C57BL PPAR gamma Endocrinology chemistry Gene Expression Regulation Hyperglycemia Dietary Supplements biology.protein GLUT2 Quercetin Anti-Obesity Agents Energy Metabolism Food Science |
| Zdroj: | Foodfunction. 6(3) |
| ISSN: | 2042-650X |
| Popis: | A diet rich in polyphenols can ameliorate some metabolic alterations associated with obesity and type 2 diabetes. Opuntia ficus-indica (OFI) is a plant rich in isorhamnetin glycosides and is highly consumed in Mexico. The purpose of this research was to determine the metabolic effect of an OFI extract on a mouse model of diet-induced obesity and in isolated pancreatic islets. OFI extract was added to a high fat (HF) diet at a low (0.3%) or high (0.6%) dose and administered to C57BL/6 mice for 12 weeks. Mice fed the HF diet supplemented with the OFI extract gained less body weight and exhibited significantly lower circulating total cholesterol, LDL cholesterol and HDL cholesterol compared to those fed the HF diet alone. The HF-OFI diet fed mice presented lower glucose and insulin concentration than the HF diet fed mice. However, the HF-OFI diet fed mice tended to have higher insulin concentration than control mice. The OFI extract stimulated insulin secretion in vitro, associated with increased glucose transporter 2 (GLUT2) and peroxisome proliferator-activated receptor gamma (PPARγ) mRNA content. Furthermore, the OFI extract improved glucose tolerance, and additionally increased energy expenditure. These metabolic improvements were associated with reduced adipocyte size, increased hepatic IRS1 tyr-608 and S6 K thr-389 phosphorylation. OFI isorhamnetin glycosides also diminished the hepatic lipid content associated with reduced mRNA expression of the endoplasmic reticulum stress markers and lipogenic enzymes and increased mRNA expression of genes related to fatty acid oxidation. Overall, the OFI extract prevented the development of metabolic abnormalities associated with diet-induced obesity. |
| Databáze: | OpenAIRE |
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