Polar surface distribution of type II insulin-like growth factor receptor in rat hepatocytes
| Autor: | Susanne Keller, Peter J. Meier, Silvia E. Jaeggi-Groisman, E.Rudolf Froesch, Brigitte Zimmerii |
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| Rok vydání: | 1990 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Receptors Cell Surface Insulin-Like Growth Factor Receptor Biology Binding Competitive Biochemistry Endocrinology Growth factor receptor Insulin-Like Growth Factor II Internal medicine medicine Animals Binding site Receptor Molecular Biology Binding Sites Growth factor Cell Membrane Rats Inbred Strains Receptors Somatomedin Rats medicine.anatomical_structure Liver Hormone receptor Hepatocyte Insulin-like growth factor 2 biology.protein |
| Zdroj: | Molecular and Cellular Endocrinology. 74:69-74 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/0303-7207(90)90206-n |
| Popis: | We describe the distribution of type II insulin-like growth factor receptors among canalicular (cLPM) and basolateral (bLPM) subfractions of rat liver plasma membranes (LPM). BLPM bound 3 times more 125I-IGF II than cLPM. The number of receptors was (1.3 +/- 0.15) X 10(-12) mol/mg in bLPM, and (0.4 +/- 0.17) X 10(-12) mol/mg in cLPM. Insulin-like growth factor II (IGF II) was 10 times more potent than insulin-like growth factor I (IGF I) in displacing 125I-IGF II from both basolateral and canalicular binding sites. Insulin did not interfere with binding of 125I-IGF II in either LPM preparations. Our findings point to an asymmetrical hepatocellular distribution of type II IGF receptors, thus extending the concept of surface polarization of hepatocytes to growth promoting hormone receptors. |
| Databáze: | OpenAIRE |
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