In vitro Antibiotic and Modulatory Activity of Mesosphaerum suaveolens (L.) Kuntze against Candida strains
| Autor: | Maria Flaviana Bezerra Morais Braga, Viviane Bezerra da Silva, João Cruz Neto, Jean Paul Kamdem, Antonia Thassya Lucas dos Santos, José Weverton Almeida Bezerra, Marcello Iriti, Sara Vitalini, Maria Audilene de Freitas, Rafael Pereira da Cruz, Maria Ivaneide Rocha, Antonia Eliene Duarte, Adrielle Rodrigues Costa, Luiz Marivando Barros, Leomara Andrade da Silva |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Phytochemistry hyptis suaveolens Enzyme-linked Immunosorbent Assay Medicinal Plant Antibiotic Agent HPLC-DAD Biochemistry High-performance liquid chromatography Catechin Disk Diffusion chemistry.chemical_compound Candida Tropicalis Hyptis suaveolens Cell Viability Caffeic acid Pharmacology (medical) Apigenin General Pharmacology Toxicology and Pharmaceutics Fluconazole Anti-microbial Activity Candida Ultraviolet Spectroscopy biology Broth microdilution Rutoside Infectious Diseases Mesosphaerum Suaveolens Extract Spectrophotometry Minimum Inhibitory Concentration Priority Journal Quercetin Chlorogenic Acid phychochemistry medicine.drug Microbiology (medical) Anti-fungal Activity bamburral 030106 microbiology Drug Activity Context (language use) Phytochemical candida Broth Dilution Microbiology Article Plant Extract Unclassified Drug 03 medical and health sciences Ellagic Acid Gallic Acid Cell Viability Assay medicine Controlled Study Phenol Derivative Fungal Strain IC50 Retention Time (chromatography) Minimum Fungicidal Concentration Chromatography lcsh:RM1-950 Nonhuman biology.organism_classification High Performance Liquid Chromatography lcsh:Therapeutics. Pharmacology 030104 developmental biology Caffeic Acid chemistry Fungus Growth Flavonoid Antifungal Resistance Ic 50 Mesosphaerum Suaveolens |
| Zdroj: | Antibiotics Antibiotics, Vol 9, Iss 2, p 46 (2020) Repositório Institucional do INPA Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA Volume 9 Issue 2 |
| ISSN: | 2079-6382 |
| DOI: | 10.3390/antibiotics9020046 |
| Popis: | The emergence of fungal resistance to commercial drugs has been a major problem for the WHO. In this context, research with natural products is promising in the discovery of new active substances. Thus, this work evaluated the antifungal effect of a medicinal plant (i.e., Mesosphaerum suaveolens) against strains of the genus Candida, tested the combined effect with the drug fluconazole, and, finally, determined the phenolic constituents present in the species. Initially, aqueous extracts of leaves (AELMs) and aerial parts (AEAPMs) of the species were prepared. For microbiological assays, the minimum fungicidal concentration was determined by broth microdilution, and the combined effect of fluconazole extracts were verified by sub-inhibitory microdilution concentrations (CFM/8) followed by spectrophotometric readings which were used to determine the IC50. HPLC detected the presence of flavonoids and phenolic acids, detecting eight compounds present in the samples of which caffeic acid and quercetin were major components. The AELMs modulated fluconazole activity since it decreased fluconazole&rsquo s IC50 from 7.8 µ g/mL to an IC50 of 4.7 µ g/mL (CA LM 77) and from 28.8 µ g/mL to 18.26 µ g/mL (CA INCQS 40006) for the C. albicans strains. The AEAPMs were able to potentiate the effect of fluconazole more effectively than the AELMs. Such an effect was significant for the 16 µ g/mL concentration for CA LM 77 and 32 µ g/mL for CA INCQS 40006. The AEAPMs as well as the AELMs presented clinically relevant activities for C. tropicalis strains. For the C. tropicalis LM 23 strain, the AEPMs obtained an IC50 of 25 µ g/mL and the AELMs an IC50 of 359.9 µ g/mL. |
| Databáze: | OpenAIRE |
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