Influences of melatonin treatment, melatonin receptor 1A (MTNR1A) and kisspeptin (KiSS-1) gene polymorphisms on first conception in Sarda ewe lambs
| Autor: | Sebastiano Luridiana, Vincenzo Carcangiu, Sara Bodano, Giovanni Cosso, F. Zidda, F Farci, Maria Consuelo Mura, Cinzia Daga |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Drug Resistance Single-nucleotide polymorphism Reproductive technology Biology Polymorphism Single Nucleotide Antioxidants Melatonin 03 medical and health sciences Endocrinology Kisspeptin Gene Frequency Internal medicine Reproductive biology Genotype Genetics medicine Animals Amplified Fragment Length Polymorphism Analysis Molecular Biology Alleles Genetic Association Studies Sheep Domestic Drug Implants Kisspeptins Receptor Melatonin MT1 Domestic sheep reproduction 0402 animal and dairy science Exons Fertility Agents Female 04 agricultural and veterinary sciences 040201 dairy & animal science 030104 developmental biology Melatonin receptor 1A Amino Acid Substitution Italy Reproductive Medicine Fertilization Female Animal Science and Zoology Live Birth Developmental Biology Biotechnology medicine.drug |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1031-3613 |
| Popis: | In order to investigate if the melatonin receptor 1A (MTNR1A) and kisspeptin (KiSS-1) genes influence the reproductive response to melatonin treatment, 510 Sarda ewe lambs were divided into groups C (control) and M; Group M received one melatonin implant (18 mg). After 35 days rams were introduced for 40 days and subsequent lambing dates and number of newborns were recorded. The MTNR1A gene Exon II and KiSS-1 gene Exon I were amplified and genotyped by restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism analysis. Two single nucleotide polymorphisms (SNPs; C606T and G612A) in MTNR1A and one (G1035A) in KiSS-1 were found. The most frequent genotypes were G/G (63%) and C/C (53%) for MTNR1A and G/G (92%) for KiSS-1. Treated animals showed a higher lambing rate (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|