Induction of micronuclei in respiratory tract following radon inhalation
Autor: | S. Bao, M. A. Khan, Antone L. Brooks, B. H. Wood, F. T. Cross, W. B. Chrisler, R. A. Gies, P. W. Harwood |
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Rok vydání: | 1997 |
Předmět: |
Male
Pathology medicine.medical_specialty Binucleated cells Biology Species Specificity medicine Dosimetry Animals Radiology Nuclear Medicine and imaging Linear Energy Transfer Rats Wistar Lung Cells Cultured Micronuclei Chromosome-Defective Radiological and Ultrasound Technology Inhalation Dose-Response Relationship Radiation Rats Trachea Nasal Mucosa medicine.anatomical_structure Radon Absorbed dose Micronucleus test Micronucleus Respiratory tract |
Zdroj: | International journal of radiation biology. 72(5) |
ISSN: | 0955-3002 |
Popis: | Male Wistar rats were exposed to radon and its progeny (0.0, 60, 262 and 564 working level months, WLM), and the frequency of micronuclei was determined in deep lung fibroblasts, and deep lung, trachea and nasal epithelial cells with slopes of 0.28, 0.67, 0.34 and 0.11 micronuclei/1000 binucleated cells/WLM respectively. Micronuclei in deep lung fibroblasts, isolated and cultured using two methods and media, demonstrated no differences in slopes. Biological damage was used as a biodosimeter to calculate the relationship between dosimetric units: alpha particle traversals or 'nuclear hits', dose in mGy and exposure in WLM. The estimated number of nuclear alpha traversals/Gy was 6.3. Radon exposure to 170 WLM resulted in the same frequency of micronuclei in deep lung epithelial cells as produced by one alpha hit/cell nucleus. Absorbed dose/unit of exposure (mGy/WLM) was estimated assuming the damage was related to absorbed dose or to changes in cell sensitivity and ranged from 1.13 to 1.34 for deep lung epithelial cells, 0.47 to 1.09 for deep lung fibroblasts, 0.34 to 0.67 for tracheal epithelial cells and 0.18 to 0.33 for nasal epithelial cells. Biological dosimetry can be used to relate exposure to damage, compare dosimetric units and validate physical dosimetry models. This approach can be applied to any inhaled material capable of producing biological damage. |
Databáze: | OpenAIRE |
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