Dietary thylakoids reduce visceral fat mass and increase expression of genes involved in intestinal fatty acid oxidation in high-fat fed rats
| Autor: | Wolfgang Langhans, Eva-Lena Stenblom, Britta Bonn, Caroline Montelius, Charlotte Erlanson-Albertsson, Emil Egecioglu, Deepti Ramachandran, Abdelhak Mansouri, Björn Weström |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Physiology Intra-Abdominal Fat Biology Diet High-Fat Thylakoids High fat fed Rats Sprague-Dawley 03 medical and health sciences Fat accumulation Physiology (medical) Internal medicine medicine Animals Intestinal Mucosa Visceral fat Beta oxidation Gene 2. Zero hunger 030109 nutrition & dietetics Fatty Acids food and beverages Lipid metabolism Organ Size Rats Up-Regulation Steatorrhea Endocrinology Thylakoid medicine.symptom Oxidation-Reduction |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 311:R618-R627 |
| ISSN: | 1522-1490 0363-6119 |
| DOI: | 10.1152/ajpregu.00212.2016 |
| Popis: | Thylakoids reduce body weight gain and body fat accumulation in rodents. This study investigated whether an enhanced oxidation of dietary fat-derived fatty acids in the intestine contributes to the thylakoid effects. Male Sprague-Dawley rats were fed a high-fat diet with ( n = 8) or without thylakoids ( n = 8) for 2 wk. Body weight, food intake, and body fat were measured, and intestinal mucosa was collected and analyzed. Quantitative real-time PCR was used to measure gene expression levels of key enzymes involved in fatty acid transport, fatty acid oxidation, and ketogenesis. Another set of thylakoid-treated ( n = 10) and control rats ( n = 10) went through indirect calorimetry. In the first experiment, thylakoid-treated rats ( n = 8) accumulated 25% less visceral fat than controls. Furthermore, fatty acid translocase ( Fat/Cd36), carnitine palmitoyltransferase 1a ( Cpt1a), and mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2 ( Hmgcs2) genes were upregulated in the jejunum of the thylakoid-treated group. In the second experiment, thylakoid-treated rats ( n = 10) gained 17.5% less weight compared with controls and their respiratory quotient was lower, 0.86 compared with 0.91. Thylakoid-intake resulted in decreased food intake and did not cause steatorrhea. These results suggest that thylakoids stimulated intestinal fatty acid oxidation and ketogenesis, resulting in an increased ability of the intestine to handle dietary fat. The increased fatty acid oxidation and the resulting reduction in food intake may contribute to the reduced fat accumulation in thylakoid-treated animals. |
| Databáze: | OpenAIRE |
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