Combined method for the determination of γ-aminobutyric and β-alanine in cerebrospinal fluid by stable isotope dilution mass spectrometry

Autor:	W.S. Guerand, C.A.J.M. Jakobs, H.J. ten Brink, E.A. Struys
Rok vydání:	1999
Předmět:	Quality Control Alanine Chromatography Chemistry Stable isotope ratio General Chemistry Reference Standards Mass spectrometry Stable isotope dilution Mass Spectrometry Cerebrospinal fluid Isotopes beta-Alanine Humans gamma-Aminobutyric Acid Combined method
Zdroj:	Journal of Chromatography B: Biomedical Sciences and Applications. 732:245-249
ISSN:	0378-4347
DOI:	10.1016/s0378-4347(99)00283-2
Popis:	A previously described method for the determination of GABA in CSF has been expanded to include both GABA and beta-ALA, using a single GC-MS analysis. A stable isotope labelled internal standard for beta-ALA was synthesised to achieve accurate quantification. This new combined method expands the diagnostic power compared to an isolated GABA measurement. Control values for free and total GABA and free and total beta-ALA are described. Age2 years: free GABA 0.017-0.067 microM, total GABA 4.2-13.4 microM; free beta-ALA 0.049-0.11 microM, total beta-ALA 2.1-4.6 microM. Age2 years: free GABA 0.032-0.17 microM, total GABA 3.3-12.2 microM; free beta-ALA 0.021-0.058 microM, total beta-ALA 0.91-3.5 microM.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce02bd237aa57f6e16d45e6f4dea7259 https://doi.org/10.1016/s0378-4347(99)00283-2 Zobrazit plný text záznamu