The roles of chemokine (C-X-C motif) ligand 13 in spinal cord ischemia-reperfusion injury in rats
| Autor: | Yongjian Zhou, Hong Ma, Zhe Li, Ziyun Qiang, Fengshou Chen, Xiao-Qian Li |
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| Rok vydání: | 2020 |
| Předmět: |
Receptors
CXCR5 0301 basic medicine Chemokine MAP Kinase Signaling System Interleukin-1beta CXCR5 Proinflammatory cytokine Rats Sprague-Dawley Mice 03 medical and health sciences Chemokine receptor 0302 clinical medicine medicine Animals RNA Small Interfering CXCL13 Extracellular Signal-Regulated MAP Kinases Molecular Biology biology Caspase 3 Interleukin-6 Spinal Cord Ischemia Tumor Necrosis Factor-alpha Kinase Chemistry General Neuroscience Chemokine CXCL13 Rats Cell biology Disease Models Animal 030104 developmental biology medicine.anatomical_structure Reperfusion Injury biology.protein Neurology (clinical) Neuron NeuN 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Brain Research. 1727:146489 |
| ISSN: | 0006-8993 |
| Popis: | Spinal cord ischemia-reperfusion injury (SCII) remains an unresolved complication and its underlying mechanism has not been fully elucidated. In this study, we studied the role of chemokine (C-X-C motif) ligand 13 (CXCL13) in a rat model of SCII. We examined the time course and cellular distribution of CXCL13 protein in rats after SCII. The effects of siRNA targeting CXCL13 or C-X-C chemokine receptor type 5 (CXCR5) in SCII were also investigated. Neurological function, histological assessment, and disruption of the blood-spinal cord barrier (BSCB) were evaluated. The expression levels of CXCL13, CXCR5, phosphorylated extracellular signal-regulated kinase (p-ERK), caspase-3, interleukin 6 (IL-6), TNF-α, and IL-1β were determined. We found that SCII resulted in impaired hind limb function and increased the expression of CXCL13. In addition, CXCL13 expression demonstrated the most pronounced effect at 24 h after SCII. We reveal that CXCL13 protein was co-expressed with the mature neuron marker NeuN and the microglial marker IBA-1 in spinal cord tissues of model rats. SCII also increased the expression of CXCR5, p-ERK, caspase-3, IL-6, TNF-α, and IL-1β at 24 h after SCII. Pre-treatment with CXCL13 siRNA protected the rats against SCII and decreased the expression of signalling pathway proteins and proinflammatory cytokines mentioned above. CXCR5 siRNA also showed similar protective effects. These findings indicate that CXCL13 is involved in SCII. The CXCL13/CXCR5 axis promotes the development of SCII, possibly via ERK-mediated pathways. Targeting the mechanism of CXCL13 involved in the development of SCII might be a potential approach for the treatment of this condition. |
| Databáze: | OpenAIRE |
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