Adipose-Derived Stem Cells Inhibited the Proliferation of Bladder Tumor Cells by S Phase Arrest and Wnt/β-Catenin Pathway
Autor: | Shenglan Li, Xiuheng Liu, Jian Lin, Cong Qin, Tao Xu, Tao Wang, Lei Wang, Xi Yu, Tao Bai |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
medicine.medical_treatment Cyclin A Adipose tissue urologic and male genital diseases 03 medical and health sciences Cyclin-dependent kinase Cell Line Tumor medicine bladder tumor Humans Wnt Signaling Pathway beta Catenin Research Articles adipose-derived stem cell Wnt/β-catenin 030102 biochemistry & molecular biology biology Stem Cells Wnt signaling pathway Cell Biology Stem-cell therapy S phase arrest Coculture Techniques Neoplasm Proteins 030104 developmental biology Adipose Tissue Urinary Bladder Neoplasms Apoptosis Catenin S Phase Cell Cycle Checkpoints caspase3/7 biology.protein Cancer research Stem cell Developmental Biology Biotechnology |
Zdroj: | Cellular Reprogramming |
ISSN: | 2152-4998 |
Popis: | Adipose-derived stem cells (ADSCs), which are present in most organs and tissues, were evaluated as a novel medium for stem cell therapy. In this study, we investigated the effects and underlying mechanisms of ADSCs in bladder tumor (BT) cells. SV-HUC, T24, and EJ cells were cultured with ADSCs and conditioned medium from ADSCs (ADSC-CM). We observed that in routine culture, ADSCs significantly inhibited the proliferation of T24 and EJ cells in a dose-dependent manner. In addition, ADSC-CM attenuated the viability of T24 and EJ cells in a dose-dependent manner. Cell cycle analysis indicated that ADSC-CM was capable of inducing T24 and EJ cells S phase arrest and downregulating the expression of CDK 1, whereas the expression of cyclin A was increased. ADSC-CM could induce apoptosis in T24 cells. The mechanism of this effect likely involved the caspase3/7 pathway and Wnt/β-catenin pathway. These findings demonstrated that ADSCs could inhibit the proliferation of BT cells via secretory factors. |
Databáze: | OpenAIRE |
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