Effects of denervation on energy metabolism of rat hindlimb muscles: application of 31P-MRS and 19F-MRS
| Autor: | Keiko Koga, Nobutaka Miki, Hiroaki Takai, Takaaki Ikata, Takayuki Sogabe, Shinjiro Takata |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Fluorine Radioisotopes medicine.medical_specialty Magnetic Resonance Spectroscopy Phosphocreatine Intracellular pH Physical Exertion Stimulation Physical exercise Isometric exercise Hindlimb Phosphates chemistry.chemical_compound Blood Substitutes Isometric Contraction Internal medicine medicine Animals Orthopedics and Sports Medicine Rats Wistar Muscle Skeletal Denervation Fluorocarbons Phosphorus Isotopes Organ Size Hydrogen-Ion Concentration Sciatic Nerve Electric Stimulation Rats Muscular Atrophy Endocrinology chemistry Regional Blood Flow Surgery Sciatic nerve Radiopharmaceuticals Energy Metabolism Follow-Up Studies |
| Zdroj: | Journal of Orthopaedic Science. 4:370-375 |
| ISSN: | 0949-2658 |
| DOI: | 10.1007/s007760050118 |
| Popis: | We studied the effects of denervation on the energy metabolism and peripheral circulation dynamics of rat hindlimb muscles during and after exercise. The sciatic nerves of male Wistar rats were cut to produce denervation. Energy metabolism was assessed by phosphorus-31 magnetic resonance spectroscopy (MRS), and circulation by fluorine-19 MRS. Exercise of rat hindlimb muscles was induced by electrical stimulation at 40 Hz. The inorganic phosphate (Pi) / ¿Pi + phosphocreatine (PCr)¿ ratio, an indicator of the energy level, was 0.795 immediately after denervation. The ratios 4 and 8 weeks after denervation were 0.870 and 0.853, respectively. The intracellular pH during the 4 min after initiation of stimulation was significantly reduced 4 and 8 weeks after denervation compared with the value immediately after denervation. The signal strength of the research perfluoro-carbon (FC-43; perfluorotributylamine) a measure of circulation dynamics, increased to 167% in controls during exercise, but an increase of only 134% was seen in rats 8 weeks after denervation. These results showed that the energy supply and circulation dynamics in denervated atrophic muscles decreased during exercise compared with findings in control muscles. |
| Databáze: | OpenAIRE |
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