VP3 is crucial for the stability of Nora virus virions
| Autor: | Venkateswara Rao Jonna, Jens-Ola Ekström, Sajna Anand Sadanandan, Dan Hultmark, Anders Hofer |
|---|---|
| Přispěvatelé: | BioMediTech - BioMediTech, University of Tampere |
| Jazyk: | angličtina |
| Předmět: |
Protein Conformation
alpha-Helical 0301 basic medicine RNA viruses Cancer Research Proteases viruses Gene Expression Genome Capsid stability Virus 03 medical and health sciences RNA Virus Infections Protein Domains Virology Virus biology Amino Acid Sequence Coiled coil biology Nora virus Virus Assembly Virion RNA virus diseases biochemical phenomena metabolism and nutrition biology.organism_classification Open reading frame 030104 developmental biology Infectious Diseases Kasvibiologia mikrobiologia virologia - Plant biology microbiology virology Capsid Mutation Capsid Proteins Protein Multimerization Drosophila melanogaster |
| Zdroj: | Virus Research. :20-27 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2016.06.011 |
| Popis: | Nora virus is an enteric virus that causes persistent, non-pathological infection in Drosophila melanogaster. It replicates in the fly gut and is transmitted via the fecal-oral route. Nora virus has a single-stranded positive-sense RNA genome, which is translated in four open reading frames. Reading frame three encodes the VP3 protein, the structure and function of which we have investigated in this work. We have shown that VP3 is a trimer that has an α-helical secondary structure, with a functionally important coiled-coil domain. In order to identify the role of VP3 in the Nora virus life cycle, we constructed VP3-mutants using the cDNA clone of the virus. Our results show that VP3 does not have a role in the actual assembly of the virus particles, but virions that lack VP3 or harbor VP3 with a disrupted coiled coil domain are incapable of transmission via the fecal-oral route. Removing the region downstream of the putative coiled coil appears to have an effect on the fitness of the virus but does not hamper its replication or transmission. We also found that the VP3 protein and particularly the coiled coil domain are crucial for the stability of Nora virus virions when exposed to heat or proteases. Hence, we propose that VP3 is imperative to Nora virus virions as it confers stability to the viral capsid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|