Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii
| Autor: | Maria Soledad Ramirez, Sabrina Montaña, Jasmine Martinez, Eugenio Jara, Robert A. Bonomo, Brettni Quinn, Nyah Rodman, Jennifer S. Fernandez, German Matias Traglia, Kori Place, Andrés Iriarte, Virginia Cantera |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Acinetobacter baumannii Iron 030106 microbiology Citric Acid Cycle Serum albumin lcsh:Medicine Serum Albumin Human HSA beta-Lactams Article beta-Lactam Resistance Microbiology Transcriptome Ciencias Biológicas purl.org/becyt/ford/1 [https] 03 medical and health sciences Biología Celular Microbiología Humans lcsh:Science purl.org/becyt/ford/1.6 [https] Gene Bacterial Capsules Type VI secretion system Regulation of gene expression Multidisciplinary Ion Transport Microbial Viability biology Gene Expression Profiling lcsh:R Biofilm Gene Expression Regulation Bacterial Type VI Secretion Systems biology.organism_classification 3. Good health Zinc Biofilms biology.protein lcsh:Q Efflux Transformation Bacterial Genes MDR Genome Bacterial CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-16 (2018) |
| Popis: | In the past few decades Acinetobacter baumannii has emerged as a notorious nosocomial pathogen because of its ability to acquire genetic material and persist in extreme environments. Recently, human serum albumin (HSA) was shown to significantly increase natural transformation frequency in A. baumannii. This observation led us to perform transcriptomic analysis of strain A118 under HSA induction to identify genes that are altered by HSA. Our results revealed the statistically significant differential expression of 296 protein-coding genes, including those associated with motility, biofilm formation, metabolism, efflux pumps, capsule synthesis, and transcriptional regulation. Phenotypic analysis of these traits showed an increase in surface-associated motility, a decrease in biofilm formation, reduced activity of a citric acid cycle associated enzyme, and increased survival associated with zinc availability. Furthermore, the expression of genes known to play a role in pathogenicity and antibiotic resistance were altered. These genes included those associated with RND-type efflux pumps, the type VI secretion system, iron acquisition/metabolism, and ß-lactam resistance. Together, these results illustrate how human products, in particular HSA, may play a significant role in both survival and persistence of A. baumannii. Fil: Quinn, Brettni. California State University; Estados Unidos Fil: Rodman, Nyah. California State University; Estados Unidos Fil: Jara, Eugenio. Universidad de la República; Uruguay Fil: Fernandez, Jennifer S.. California State University; Estados Unidos Fil: Martinez, Jasmine. California State University; Estados Unidos Fil: Traglia, German Matias. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Cantera, Virginia. Universidad de la República; Uruguay Fil: Place, Kori. California State University; Estados Unidos Fil: Bonomo, Robert A.. Case Western Reserve University; Estados Unidos. Louis Stokes Cleveland Department of Veterans Affair; Estados Unidos Fil: Iriante, Andrés. Universidad de la República; Uruguay Fil: Ramirez, María Soledad. California State University; Estados Unidos |
| Databáze: | OpenAIRE |
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