Histone H1 deposition and histone-DNA interactions in replicating chromatin
| Autor: | Andrei D. Mirzabekov, Jordanka Zlatanova, Todor Banchev, Ljuba Srebreva, Roumen Tsanev, Sergei Bavykin |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
DNA Replication
Solenoid (DNA) Sulfuric Acid Esters Biology Cell Fractionation Histones Mice Histone H1 Histone H2A Histone methylation Tumor Cells Cultured Animals Histone code Electrophoresis Gel Two-Dimensional Histone octamer Carcinoma Ehrlich Tumor Cell Nucleus Multidisciplinary DNA Neoplasm Chromatin Cell biology Cross-Linking Reagents Bromodeoxyuridine Biochemistry Histone methyltransferase Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 90:3918-3922 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | An immunochemical method for analyzing protein interactions with BrdUrd-substituted DNA was used to study binding of histones to nascent DNA in nuclei. The results indicate that in Ehrlich ascites tumor (EAT) cells, histone H1 deposits on newly replicated DNA simultaneously with or immediately after core histone deposition so that in chromatin replicated for 3 min, the stoichiometry of the histones is the same as in bulk chromatin. All histones, and especially histone H1, interact with nascent DNA more weakly than with bulk chromatin, although the efficiency of interaction via the globular domains seems to be the same for both types of chromatin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|