A BAC-based physical map of the major autosomes of Drosophila melanogaster
| Autor: | Mohammed Naeemuddin, Pieter J. de, Jong, James Durbin, Steven E. Scherer, Roger A. Hoskins, Paul E. Tabor, Michael R. Bailey, Joseph J. Catanese, Robert G. David, Susan E. Celniker, Lisa Ciesiolka, Catherine R. Nelson, Andrew D. Arenson, Kazutoyo Osoegawa, Richard A. Gibbs, Aaron G. Mammoser, Denise R. DeShazo, Benjamin P. Berman, Todd R. Laverty, Gerald M. Rubin, Reed A. George |
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| Rok vydání: | 2000 |
| Předmět: |
Genetic Markers
Sequence analysis Centromere Genetic Vectors Restriction Mapping Sequence assembly Genes Insect Computational biology Biology Contig Mapping Sequence-tagged site Euchromatin Restriction map Animals Cloning Molecular In Situ Hybridization Gene Library Repetitive Sequences Nucleic Acid Sequence Tagged Sites Genetics Bacterial artificial chromosome Multidisciplinary Polytene chromosome Genome Contig Sequence Analysis DNA Chromosomes Bacterial Telomere DNA Fingerprinting Chromatin Drosophila melanogaster |
| Zdroj: | Science (New York, N.Y.). 287(5461) |
| ISSN: | 0036-8075 |
| Popis: | We constructed a bacterial artificial chromosome (BAC)–based physical map of chromosomes 2 and 3 of Drosophila melanogaster , which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing. |
| Databáze: | OpenAIRE |
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