Grinding Lysis (GL): A microfluidic device for sample enrichment and mechanical lysis in one
| Autor: | Valentin Flegeau, Jérome Ventosa, Guillaume Delapierre, Remco den Dulk, Jean Berthier, Mélanie Flaender, Anne-Gaelle Bourdat |
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| Přispěvatelé: | Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Département Microtechnologies pour la Biologie et la Santé (DTBS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), French inter-ministerial CBRNe R&D Program |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Spores Lysis Sample (material) Microfluidics Sample preparation 01 natural sciences Grinding lysis (GL) law.invention 03 medical and health sciences [SPI]Engineering Sciences [physics] law Materials Chemistry Electrical and Electronic Engineering Instrumentation Filtration Purification Chromatography Bacteria Chemistry 010401 analytical chemistry Metals and Alloys Contamination Condensed Matter Physics 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Grinding 030104 developmental biology PCR Scientific method |
| Zdroj: | Sensors and Actuators B: Chemical Sensors and Actuators B: Chemical, Elsevier, 2018, 258, pp.148-155. ⟨10.1016/j.snb.2017.11.082⟩ Sensors and Actuators B: Chemical, 2018, 258, pp.148-155. ⟨10.1016/j.snb.2017.11.082⟩ |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2017.11.082⟩ |
| Popis: | International audience; Rapid identification of health threatening bacteria and/or spores present in small concentration in sample fluids is of utmost importance. Efficient sample preparation and molecular detection aims to achieving this goal. Two processes must be conducted successively: the concentration of the targets in a small volume with simultaneous purification, followed by their lysis to provide accessible DNA templates. Conventional PCR is then used in situ to identify the targets. In this work we present an original approach combining an efficient concentration and purification of the bacteria and spores, a rapid and efficient grinding lysis step, working even for polluted samples, and the integration of the process in a semi-automated device. The method is very efficient and rapid: it can concentrate and detect less than 10 targets in 1 mL of sample, even if the sample is contaminated by some environmental contaminants. The most resistant spores are successfully lysed. In this study, we successively present the principle and performances of the method, and its integration on a in a semi-automated device. Perspectives to fully integrated system are discussed. |
| Databáze: | OpenAIRE |
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