miR-22 promotes apoptosis of osteosarcoma cells via inducing cell cycle arrest
| Autor: | Pengzhou Gai, Hongliang Sun, Lei Jiang, Zuofu Zhang, Guangda Wang, Xiaojun Qi, Qiang Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research miR-22 Biology Flow cytometry 03 medical and health sciences 0302 clinical medicine osteosarcoma medicine MTT assay Viability assay medicine.diagnostic_test cell apoptosis Cell growth Articles Cell cycle Molecular biology Cell biology 030104 developmental biology cell proliferation Oncology Apoptosis Cell culture cell cycle arrest 030220 oncology & carcinogenesis A431 cells |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| Popis: | To study the effects of miR-22 on the proliferation and the apoptosis of osteosarcoma MG-63 cell line and to explore the potential molecular mechanism that miR-22 regulates this biological process. Quantitive real-time polymerase chain reaction (RT-qPCR) was performed to explore the miRNA level of miR-22. The MG-63 cell line was infected with miR-22 mimics for establishment of miR-22 overexpression. Non-infected cells were in blank group and cells infected with empty vector were served as negative control (NC group). MTT assay was conducted to measure cell viability. The cell cycle and apoptosis were explored using flow cytometry and the apoptosis-related markers were detected by western blotting. RT-qPCR results revealed that the miR-22 miRNA level in the MG-63 cells was significantly lower than that in osteoblasts (P |
| Databáze: | OpenAIRE |
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