The development and validation of methods for evaluating the immune system in preweaning piglets
| Autor: | Brinda Mahadevan, Mark Cameron, Brandon M. Zeigler, Keith Nelson, Myra L. Weiner, Bjorn A. Thorsrud, Kristi Bailey |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Michigan Sus scrofa Positive control Immunotoxicology Toxicology Research model Immunophenotyping Immune system Toxicity Tests Medicine Animals Immunity Mucosal Crosses Genetic Innate immune system Mucous Membrane business.industry Protein Stability Models Immunological Reproducibility of Results General Medicine Flow Cytometry Immunohistochemistry Sample stability Immunity Innate Gastrointestinal Tract Animals Newborn Immunology Cytokines Gastrointestinal tissue Female business Biomarkers Food Science |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 84 |
| ISSN: | 1873-6351 |
| Popis: | The preweaning piglet has been found to be a valuable research model for testing ingredients used in infant formula. As part of the safety assessment, the neonates' immune system is an important component that has to be evaluated. In this study three concurrent strategies were developed to assess immune system status. The methods included (1) immunophenotying to assess circulating innate immune cell populations, (2) monitoring of circulating cytokines, particularly in response to a positive control agent, and (3) monitoring of localized gastrointestinal tissue cytokines using immunohistochemistry (IHC), particularly in response to a positive control agent. All assays were validated using white papers and regulatory guidance within a GLP environment. To validate the assays precision, accuracy and sample stability were evaluated as needed using a fit for purpose approach. In addition animals were treated with proinflammtory substances to detect a positive versus negative signal. In conclusion, these three methods were confirmed to be robust assays to evaluate the immune system and GIT-specific immune responses of preweaning piglets. |
| Databáze: | OpenAIRE |
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