Effects of a polyclonal antiserum to rat growth hormone on circulating insulin-like growth factor (IGF)-I and IGF-binding protein concentrations and the growth of muscle and bone
| Autor: | E Tonner, Nigel Loveridge, D J Flint, Robert M. Palmer, B M Thomson, S. C. Mackie |
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| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Hindlimb Biology Muscle Development Insulin-like growth factor-binding protein Insulin-like growth factor Endocrinology Internal medicine medicine Animals Insulin-Like Growth Factor I Rats Wistar Antiserum Bone Development Growth factor Immune Sera Body Weight Somatomedin Growth Inhibitors Rats Insulin-Like Growth Factor Binding Proteins Polyclonal antibodies Growth Hormone biology.protein Female Carrier Proteins hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0022-0795 |
| Popis: | A polyclonal antiserum to rat GH (anti-rGH) injected into rats for 3 or 8 weeks markedly reduced the weight, total protein and RNA content of muscles of the hind limb. These effects were prevented when bovine GH (bGH) was administered simultaneously. In a second experiment, the effects of 8 weeks of treatment with anti-rGH on the growth of the whole body, muscle and bone were investigated. Body weights of rats were decreased by 58% by treatment with anti-rGH; muscle weights were reduced by slightly more than the decrease in body weight (by 64%, 65% and 61% respectively for plantaris, soleus and gastrocnemius). The weight of the tibia was decreased by 54%, its length was decreased by 23%, cortical width and overall width were reduced by 26% and 18% respectively, suggesting a possible role for GH in osteoclastic activity. Serum total insulin-like growth factor-I (IGF-I) concentrations were decreased by 80–90% in both experiments by anti-rGH; these changes were prevented in the first experiment by concurrent treatment with anti-rGH and bGH. The serum IGF-binding protein-3 (IGFBP-3) concentration was also decreased by anti-rGH in experiment 1 (by 86%); the response of the 28-32 kDa IGFBPs was smaller (−35%), and was restored to control values by simultaneous injection of bGH. Western immunoblotting using an antiserum to IGFBP-2 showed that there was a marked decrease from neonatal to adult stages which was independent of anti-rGH treatment. This clearly demonstrated a dissociation of the reciprocal relationship supposed to exist between IGFBPs-2 and -3. The 24 kDa IGFBP-4 was unaffected by anti-rGH but replacement therapy with bGH doubled its concentration. Although the effects on body and muscle weight were prevented when rats were given anti-rGH and bGH simultaneously, the possibility of mediation by other hormones cannot be precluded. Journal of Endocrinology (1994) 142, 85–91 |
| Databáze: | OpenAIRE |
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