Thermopressurized diluted phosphoric acid pretreatment of ligno(hemi)cellulose to make free sugars and nutraceutical oligosaccharides
| Autor: | Marcela Tiboni, Gizele Rejane Baldo, Julia A. Kornfield, Edson Flausino Dias, José D. Fontana, Adelia Grzybowski, Robert D. Tanner |
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| Rok vydání: | 2014 |
| Předmět: |
Hot Temperature
Bioconversion Oligosaccharides Bioengineering Lignin Applied Microbiology and Biotechnology chemistry.chemical_compound Hydrolysis Polysaccharides Pressure Phosphoric Acids Hemicellulose Food science Cellulose Phosphoric acid Probiotics Monosaccharides Pinus Wood Saccharum Lactobacillus chemistry Biochemistry Biofuels visual_art Fermentation visual_art.visual_art_medium Carbohydrate Metabolism Bifidobacterium Sawdust Bagasse Biotechnology |
| Zdroj: | Journal of Industrial Microbiology and Biotechnology. 41:957-964 |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1007/s10295-014-1426-3 |
| Popis: | Ligno(hemi)cellulosics (L(h)Cs) as sugarcane bagasse and loblolly pine sawdust are currently being used to produce biofuels such as bioethanol and biobutanol through fermentation of free sugars that are often obtained enzymatically. However, this bioconversion requires a pretreatment to solubilize the hemicellulose fractions, thus facilitating the action of the cellulolytic enzymes. Instead of the main free monosaccharides used in these current models, the modulation of thermopressurized orthophosphoric acid as a pretreatment, in the ranges of 3–12 atm and pH 1.5–2.5, can produce nondigestible oligosaccharides (NDOS) such as xylo-oligosaccharides (XOS) because heteroxylan is present in both types of hardwood and softwood hemicelluloses. A comparative thin-layer chromatographic analysis of the hydrolytic products showed the best conditions for NDOS production to be 7 atm/water, pH 2.25 and 2.50, and 8.5 atm/water for both sources. Particular hydrolysates from 7 atm (171 °C) at pHs 2.25 and 2.50 both for cane bagasse and pine sawdust, with respective oligosaccharide contents of 57 and 59 %, once mixed in a proportion of 1:1 for each plant source, were used in vitro as carbon sources for Bifidobacterium or Lactobacillus. Once both bacteria attained the stationary phase of growth, an unforeseen feature emerged: the preference of B. animalis for bagasse hydrolysates and, conversely, the preference of L. casei for pine hydrolysates. Considering the fact that nutraceutical oligosaccharides from both hemicelluloses correspond to higher value-added byproducts, the technology using a much diluted thermopressurized orthophosphoric acid pretreatment becomes an attractive choice for L(h)Cs. |
| Databáze: | OpenAIRE |
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