Establishment of permanent cell lines purified from human mesothelioma: morphological aspects, new marker expression and karyotypic analysis

Autor: John-Henri Robert, Jean-Claude Pache, Sophie Dahoun, Anastase Spiliopoulos, Jacques Mauël, Marie-Marthe Philippeaux, Marc Barnet
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Mesothelioma
Cell type
Pathology
medicine.medical_specialty
Histology
Pleural Neoplasms
Simian virus 40/genetics/isolation & purification
Cell
Cell Culture Techniques
Cell Culture Techniques/methods
Vimentin
Simian virus 40
ddc:616.07
Polymerase Chain Reaction
Cytokeratin
Pleural Effusion
Malignant/genetics/metabolism/pathology
Pleural Neoplasms/genetics/metabolism/pathology
Cell Line
Tumor
medicine
Biomarkers
Tumor
DNA
Viral/analysis
Humans
ddc:576.5
Polyomavirus Infections/complications
Fluorescent Antibody Technique
Indirect
Molecular Biology
Cell Proliferation
Polyomavirus Infections
Tumor Virus Infections/complications
biology
ddc:617
Cell growth
Cell Biology
Flow Cytometry
Pleural Effusion
Malignant
Medical Laboratory Technology
Tumor Virus Infections
medicine.anatomical_structure
Cell culture
Mesothelioma/genetics/metabolism/pathology
Karyotyping
DNA
Viral
biology.protein
Epithelioid cell
Mesothelial Cell
Tumor Markers
Biological/metabolism
Zdroj: Histochemistry and Cell Biology, Vol. 122, No 3 (2004) pp. 249-60
ISSN: 0948-6143
Popis: This study reports the establishment of three major subtypes of human mesothelioma cells in tissue culture, i.e. the epithelioid, sarcomatoid and biphasic forms, and compares their phenotypic and biological characteristics. Primary cells isolated from biopsies or pleural exudates were subcultured for over 50 passages. We evaluated immunoreactivity using various mesothelial markers related to histological patterns of these cell lines. For epithelioid cells, calretinin and cytokeratin were found to be useful and easily interpretable markers as for control mesothelial cells. The biphasic form was only partially positive and the sarcomatoid type negative. Vimentin was expressed by all cell lines. BerEP4, a specific marker for adenocarcinoma, was negative. Interestingly, while the macrophage marker CD14 was negative, immunoreactivity for a mature macrophage marker (CD68) was expressed by all cell types, suggesting that this marker might constitute an additional tool useful in the differential diagnosis of mesothelioma. At the ultrastructural level, a cell surface rich in microvilli confirmed their mesothelial origin. PCR analysis revealed that none of the cell lines contained SV40 DNA. Karyotypic analyses showed more complex abnormalities in the epithelioid subtype than in the sarcomatoid form. These cell lines may be useful in the study of cellular, molecular and genetic aspects of the disease.
Databáze: OpenAIRE
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