Fyn Regulates Adipogenesis by Promoting PIKE-A/STAT5a Interaction
| Autor: | Seran Yang, Chi Bun Chan, Keqiang Ye, Margaret C.L. Tse, Xia Liu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Mice Obese Nerve Tissue Proteins Biology Proto-Oncogene Proteins c-fyn environment and public health GTP Phosphohydrolases Mice FYN GTP-Binding Proteins 3T3-L1 Cells STAT5 Transcription Factor Animals Humans Phosphorylation Kinase activity Molecular Biology Transcription factor Adipogenesis Janus kinase 2 Kinase GTPase-Activating Proteins Gene Expression Regulation Developmental hemic and immune systems Articles Cell Biology Janus Kinase 2 enzymes and coenzymes (carbohydrates) HEK293 Cells Adipose Tissue embryonic structures biology.protein Cancer research biological phenomena cell phenomena and immunity Tyrosine kinase |
| Zdroj: | Molecular and Cellular Biology. 33:1797-1808 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.01410-12 |
| Popis: | Fyn is a tyrosine kinase with multiple roles in a variety of cellular processes. Here we report that Fyn is a new kinase involved in adipocyte differentiation. Elevated Fyn protein is detected specifically in the adipocytes of obese mice. Moreover, Fyn expression increases progressively in 3T3-L1 cells during in vitro adipogenesis, which correlates with its kinase activity. Inhibition of Fyn by either genetic or pharmacological manipulation restrains the 3T3-L1 preadipocytes from fully differentiating into mature adipocytes. Mechanistically, Fyn regulates the activity of the adipogenic transcription factor signal transducer and activator of transcription 5a (STAT5a) through enhancing its interaction with the GTPase phosphoinositide 3-kinase enhancer A (PIKE-A). The STAT5a activity is therefore reduced in Fyn- or PIKE-ablated adipose tissues, leading to diminished expression of adipogenic markers and adipocyte differentiation. Our data thus demonstrate a novel functional interaction between Fyn, PIKE-A, and STAT5a in mediating adipogenesis. |
| Databáze: | OpenAIRE |
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