Modulating short wavelength fluorescence with long wavelength light
| Autor: | Michelle M. Paquette, Paul A. Liddell, Graeme Copley, Laimonas Kelbauskas, Natia L. Frank, Devens Gust, Brian R. Cherry, Christopher L. Gray, Gerdenis Kodis, Jeffrey Crisman, Thomas A. Moore, Jason G. Gillmore, Benjamin D. Sherman, Ana L. Moore |
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| Rok vydání: | 2014 |
| Předmět: |
Fluorophore
Indoles Light Population medicine.disease_cause Photochemistry Biochemistry Catalysis Photochromism chemistry.chemical_compound Colloid and Surface Chemistry Oxazines medicine Benzopyrans Spiro Compounds education Fluorescent Dyes education.field_of_study Quenching (fluorescence) Molecular Structure Chemistry Absorption Radiation General Chemistry Electrochemical Techniques Photochemical Processes Fluorescence Wavelength Models Chemical Excited state Ultraviolet |
| Zdroj: | Journal of the American Chemical Society. 136(34) |
| ISSN: | 1520-5126 |
| Popis: | Two molecules in which the intensity of shorter-wavelength fluorescence from a strong fluorophore is modulated by longer-wavelength irradiation of an attached merocyanine-spirooxazine reverse photochromic moiety have been synthesized and studied. This unusual fluorescence behavior is the result of quenching of fluorophore fluorescence by the thermally stable, open, zwitterionic form of the spirooxazine, whereas the photogenerated closed, spirocyclic form has no effect on the fluorophore excited state. The population ratio of the closed and open forms of the spirooxazine is controlled by the intensity of the longer-wavelength modulated light. Both square wave and sine wave modulation were investigated. Because the merocyanine-spirooxazine is an unusual reverse photochrome with a thermally stable long-wavelength absorbing form and a short-wavelength absorbing photogenerated isomer with a very short lifetime, this phenomenon does not require irradiation of the molecules with potentially damaging ultraviolet light, and rapid modulation of fluorescence is possible. Molecules demonstrating these properties may be useful in fluorescent probes, as their use can discriminate between probe fluorescence and various types of adventitious "autofluorescence" from other molecules in the system being studied. |
| Databáze: | OpenAIRE |
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