Expression profiles of immunity and reproductive genes during transition period in Holstein cattle
| Autor: | Wael Eldomany, Hanaa M Ghanem, Yousef Y. Elseady, Hussein, Rasha M. Saleh, Ahmed Ateya |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
beta-Defensins 040301 veterinary sciences Ice calving Biology 0403 veterinary science Transcriptome Andrology 03 medical and health sciences Endocrinology Immune system Immunity Stress Physiological Peripartum Period Animals RNA Messenger Insulin-Like Growth Factor I Gene Immunosuppression Therapy Toll-Like Receptors 04 agricultural and veterinary sciences Prolactin TLR2 030104 developmental biology Biomarker (medicine) Animal Science and Zoology Cattle Female Biomarkers Biotechnology |
| Zdroj: | Reproduction in domestic animals = Zuchthygiene. 53(2) |
| ISSN: | 1439-0531 |
| Popis: | The transition period is a critical time for dairy cows as the animal is subjected to the physiological stress accompanying parturition. Immunosuppression and health status were examined during this period in 80 Holstein cows. Blood samples were taken from each cow 3, 2 and 1 week before and after calving, and at calving (0 day). RNA was extracted and subjected to real-time PCR to determine mRNA levels for the immune-related genes TLR 2, 4, 6, 7 and β-defensin 5 in addition to the reproduction-related genes prolactin and IGF-I. Results showed significant up-regulation of pro-inflammatory-selected genes, TLR 4, 6 7 and β-defensin 5 at the third-week post-calving; however, earlier periods had lower expression of such genes. In contrast, the immunosuppression biomarker TLR2 gene was up-regulated at calving and 1 week after parturition and then down-regulated again at second and third week. Prolactin and IGF-I genes expression levels were significantly and gradually increased mainly post-partum. This research highlights that the expression patterns of TLRs, BNBD5, PRL and IGF-I could be biomarkers to follow up immune and reproductive status of dairy cow at peri-parturient period to predict the most susceptible risk time for disease incidence and to build up management protocol. |
| Databáze: | OpenAIRE |
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