Expression of insulin-like growth factor receptors in primary human thyroid neoplasms
| Autor: | Hitomi Murakami, Yoshito Ohba, Kazuo Shizume, Takao Obara, Yoshihide Fujimoto, Tohru Yashiro, Kunihiko Ito, Toshio Tsushima |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Adenoma
Adult Male medicine.medical_specialty Adolescent Endocrinology Diabetes and Metabolism medicine.medical_treatment Thyroid Gland Receptors Cell Surface Insulin-Like Growth Factor Receptor Thyroid hormone receptor beta Endocrinology Somatomedins Internal medicine medicine Humans Thyroid Neoplasms Insulin-Like Growth Factor I Receptor Thyroid cancer Aged Thyroid hormone receptor business.industry Insulin Carcinoma Thyroid Receptors Somatomedin General Medicine Middle Aged medicine.disease Somatomedin Carcinoma Papillary medicine.anatomical_structure Autoradiography Electrophoresis Polyacrylamide Gel Female business |
| Zdroj: | Acta Endocrinologica. 121:112-120 |
| ISSN: | 1479-683X 0804-4643 |
| DOI: | 10.1530/acta.0.1210112 |
| Popis: | The presence of IGF-I receptors was demonstrated in normal and neoplastic tissues of human thyroid. Binding of [125I]IGF-I to thyroid membranes was dependent on time and temperature of incubation, and maximal binding was achieved at 4°C and 18 h of incubation. [125I] IGF-I binding was dose-dependently displaced by unlabelled IGF-I; half-maximal inhibition occurred at concentrations of 10–20 μg/l. IGF-II and insulin had relative potencies of 5 and 1% compared with IGF-I. Scatchard analysis of binding data revealed a single class of IGF-I receptors with high affinity (Ka: 1.2–8.6 × 109 1/mol) in normal thyroid tissues. Affinity cross-linking and autoradiography demonstrated the type I IGF receptors. Specific binding of [125I] IGF-I in thyroid cancer tissues (9.69 ± 2.07% per 200 μg protein; mean ± sem, N = 8) was significantly (p |
| Databáze: | OpenAIRE |
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