Detection of a divergent variant of grapevine virus F by next-generation sequencing
| Autor: | Johan T. Burger, Nicholas Molenaar, Hans J. Maree |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Whole genome sequencing
Genetics Sanger sequencing Sequence Analysis RNA Grapevine virus F Molecular Sequence Data Genetic Variation High-Throughput Nucleotide Sequencing Genomics Genome Viral General Medicine Genome project Biology Genome DNA sequencing Open Reading Frames South Africa symbols.namesake Virology symbols Flexiviridae Vitis Phylogeny Plant Diseases Reference genome |
| Zdroj: | Archives of Virology. 160:2125-2127 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s00705-015-2466-3 |
| Popis: | The complete genome sequence of a South African isolate of grapevine virus F (GVF) is presented. It was first detected by metagenomic next-generation sequencing of field samples and validated through direct Sanger sequencing. The genome sequence of GVF isolate V5 consists of 7539 nucleotides and contains a poly(A) tail. It has a typical vitivirus genome arrangement that comprises five open reading frames (ORFs), which share only 88.96 % nucleotide sequence identity with the existing complete GVF genome sequence (JX105428). |
| Databáze: | OpenAIRE |
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