Tyrosine-Templated Dual-Component Silver Nanomaterials Exhibit Photoluminescence and Versatile Antimicrobial Properties through ROS Generation
| Autor: | Preeti Sagarika, Saptarshi Mukherjee, Chandan Sahi, Saurabh Rai, Subhajit Chakraborty |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Antifungal Agents
Silver Materials science Reducing agent Metal Nanoparticles Microbial Sensitivity Tests Saccharomyces cerevisiae Silver nanoparticle Nanomaterials chemistry.chemical_compound Bacillus cereus Candida albicans Escherichia coli General Materials Science Luminescent Agents Singlet Oxygen biology Singlet oxygen Ligand (biochemistry) Antimicrobial biology.organism_classification Combinatorial chemistry Small molecule Anti-Bacterial Agents chemistry Tyrosine |
| Zdroj: | ACS Applied Materials & Interfaces. 13:36938-36947 |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | The role of small molecules in the preparation of metal nanomaterials generates considerable interest in the fields from materials science to interdisciplinary sciences. In this study, a small amino acid, l-tyrosine (Tyr), has been used as a ligand precursor for the preparation of silver nanomaterials (AgNMs) comprising a dual system: smaller silver nanoclusters (responsible exclusively for the photophysical properties) and larger silver nanoparticles (responsible exclusively for the antimicrobial properties). The luminescent properties of this AgNM system substantiate the role played by Tyr as a capping and a reducing agent outside the protein environment. An interesting feature of this report is the promising antimicrobial properties of the AgNMs against Saccharomyces cerevisiae, Candida albicans, Escherichia coli, and Bacillus cereus cell lines. The importance of this work is that this investigation demonstrates the combating ability of our AgNM system against pathogenic strains (C. albicans and B. cereus) as well. Moreover, the mechanistic aspects of the antimicrobial activity of the AgNMs were elucidated using various methods, such as propidium iodide staining, monitoring reactive oxygen species generation, leakage of proteins, DNA cleavage, etc. We propose that AgNM-mediated cytotoxicity in S. cerevisiae stems from the generation of singlet oxygen (1O2) species that create oxidative stress, disrupting the cell membrane and thereby resulting in leakage of proteins from the cells. This study can pave the way toward elucidating the role of a small molecule, Tyr, in the formation of NMs and describes the use of new NMs in potential antimicrobial applications. |
| Databáze: | OpenAIRE |
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