Chitosan oligosaccharide ameliorates acute lung injury induced by blast injury through the DDAH1/ADMA pathway

Autor: Cang-Ci Tong, Peifang Cong, Yubiao Zhang, Ming-Xiao Hou, Ying Liu, Hongxu Jin, Zhou Tong, Lin Shi, Xiuyun Shi, Yun-En Liu
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Physiology
Protein Expression
lcsh:Medicine
Apoptosis
medicine.disease_cause
Pathology and Laboratory Medicine
p38 Mitogen-Activated Protein Kinases
Mice
Heart Rate
Blast Injuries
Immune Physiology
Medicine and Health Sciences
Enzyme-Linked Immunoassays
lcsh:Science
Immune Response
chemistry.chemical_classification
Innate Immune System
Multidisciplinary
Cell Death
Chemistry
Animal Models
respiratory system
Experimental Organism Systems
Cell Processes
Cytokines
Tumor necrosis factor alpha
Inflammation Mediators
Research Article
p38 mitogen-activated protein kinases
Immunology
Acute Lung Injury
Cardiology
Mouse Models
Enzyme-Linked Immunosorbent Assay
Lung injury
Research and Analysis Methods
Amidohydrolases
03 medical and health sciences
Signs and Symptoms
Model Organisms
Diagnostic Medicine
medicine
Gene Expression and Vector Techniques
Animals
Protein kinase A
Molecular Biology Techniques
Immunoassays
Molecular Biology
Inflammation
Reactive oxygen species
Molecular Biology Assays and Analysis Techniques
Chitosan
lcsh:R
Biology and Life Sciences
Cell Biology
Molecular Development
Molecular biology
respiratory tract diseases
Oxidative Stress
030104 developmental biology
Terminal deoxynucleotidyl transferase
Immune System
Immunologic Techniques
lcsh:Q
Oxidative stress
Developmental Biology
Zdroj: PLoS ONE, Vol 13, Iss 2, p e0192135 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Objective To investigate the protective effect of chitosan oligosaccharide (COS) on acute lung injury (ALI) caused by blast injury, and explore possible molecular mechanisms. Methods A mouse model of blast injury-induced ALI was established using a self-made explosive device. Thirty mice were randomly assigned to control, ALI and ALI + COS groups. An eight-channel physiological monitor was used to determine the mouse physiological index. Enzyme linked immunosorbent assay was used to measure serum inflammatory factors. Hematoxylin-eosin staining, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, immunofluorescence staining, real time-polymerase chain reaction and western blot assay were used to detect inflammatory reactions, oxidative stress and apoptosis. Results Mice were sacrificed 24 hours after successful model induction. Compared with the ALI group, the heart rate, respiration and PCO2 were significantly lower, but the PO2, TCO2 and HCO3- were significantly higher in the ALI + COS group. Compared to ALI alone, COS treatment of ALI caused a significant decrease in the wet/dry lung weight ratio, indicating a reduction in lung edema, inflammatory cell infiltration, levels of tumor necrosis factor-α, interleukin (IL)-1β, IL-4, IL-6 and nuclear factor kappa B mRNA and protein expression were reduced and IL-10 mRNA and protein expression was increased (P < 0.05). COS significantly inhibited reactive oxygen species, MDA5 and IREα mRNA and protein expressions, cell apoptosis and Bax and Caspase-3 mRNA and protein expressions, and significantly increased superoxide dismutase-1 mRNA expression, and Bcl-2 and Caspase-8 mRNA and protein expression (all P
Databáze: OpenAIRE
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