Expression of Class II Cytokine Genes in Children’s Skin
| Autor: | Sulev Kõks, Paula Reemann, Kristi Abram, Külli Kingo, Orm Porosaar, Siim Suutre, Maarjaliis Paavo, Ulvi Loite, Ene Reimann, Helgi Silm, Eero Vasar |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Biopsy Dermatology Real-Time Polymerase Chain Reaction Interleukin 10 receptor alpha subunit Interleukin 22 03 medical and health sciences Young Adult 0302 clinical medicine Immune system Medicine Humans RNA Messenger Receptor Child Cells Cultured 030304 developmental biology Aged Skin Regulation of gene expression Aged 80 and over 0303 health sciences integumentary system business.industry Interleukins Age Factors Interleukin Infant General Medicine Receptors Interleukin Middle Aged Immunohistochemistry 3. Good health Interleukin 10 Real-time polymerase chain reaction Gene Expression Regulation Child Preschool Immunology business 030215 immunology |
| Zdroj: | Acta Dermato Venereologica |
| ISSN: | 0001-5555 |
| DOI: | 10.2340/00015555-1717 |
| Popis: | Immune regulation of the skin plays an important role in susceptibility and development of illnesses. The aim of our study was to localise the interleukin (IL)-10 family of cytokines, in children's skin and to determine possible age-related differences in the expression level. The mRNA expression level of IL10, IL19, IL20, IL22, IL24, IL26, IL28B, IL29 and their receptors IL10RA, IL10RB, IL20RA, IL20RB, IL22RA1, IL22RA2, IL28RA was compared in skin biopsies of children and adults and in childrens' skin cells by quantitative real-time PCR (qRT-PCR). Immunohistochemistry was performed to confirm the qRT-PCR findings. We found age-related differences in the expression of IL10RB, IL20, IL20RA, IL22RA1, IL22RA2, IL26 and IL28RA genes. Cell type-dependent expression of IL10 family cytokines was apparent in the skin. In addition to previously known differences in systemic immunological response of adults and children, the present results reveal differences in immune profile of adult and juvenile skin. |
| Databáze: | OpenAIRE |
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