Sialylated Human Apolipoprotein E (apoEs) Is Preferentially Associated with Neuron-Enriched Cultures from APOE Transgenic Mice
| Autor: | Allen D. Roses, Donald E. Schmechel, John R. Gilbert, Michael H. Herbstreith, M. E. Eyster, Pu Ting Xu, Tracie Rothrock-Christian, H. Qiu |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Genetically modified mouse
Apolipoprotein E Blotting Western Immunocytochemistry Gene Expression Neuraminidase Mice Transgenic Nerve Tissue Proteins In situ hybridization Biology lcsh:RC321-571 Mice Apolipoproteins E Western blot medicine Animals Humans RNA Messenger lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Cells Cultured In Situ Hybridization Brain Chemistry Neurons medicine.diagnostic_test Human brain Precipitin Tests Molecular biology N-Acetylneuraminic Acid Blot medicine.anatomical_structure Neurology lipids (amino acids peptides and proteins) Neuron Neuroglia |
| Zdroj: | Neurobiology of Disease, Vol 6, Iss 1, Pp 63-75 (1999) |
| Popis: | Mice transgenic for human APOE2, E3, and E4 alleles express native 34-kDa human apoE and two sialylated apoE isoproteins with approximate molecular weights of 37 kDa (apoEs) and 39 kDa (apoEs2) in brain. These multiple apoE/apoEs/apoEs2 band patterns on Western blot are also observed in human brain, but are not seen in wild-type mouse brain. Both the 37-kDa apoEs and 39-kDa apoEs2 are coprecipitated with native 34-kDa apoE by antibody to human apoE. Neuraminidase digestion eliminates the 37- and 39-kDa forms and results in a downward shift in the bands to the position of the 34-kDa native form. These sialylated apoE isoproteins are found preferentially associated with neurons and contribute significantly (50-60%) to the total neuronal apoE in neuronal cultures from transgenic mice, while only 5-10% of total apoE is sialylated in cultures enriched in glial cells. In situ hybridization and immunocytochemistry demonstrate apoE mRNA and apoE immunoreactivity are predominantly located in cell soma of neurons, not in neuronal processes. |
| Databáze: | OpenAIRE |
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