Effect of Antioxidants on Hypoxia/Reoxygenation-Induced Injury in Isolated Perfused Rat Liver
| Autor: | Maged Younes, E. Kayser, Otfried Strubelt |
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| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Antioxidant Health Toxicology and Mutagenesis medicine.medical_treatment chemistry.chemical_element Ascorbic Acid Calcium Toxicology medicine.disease_cause Antioxidants Lipid peroxidation chemistry.chemical_compound Oxygen Consumption Glutamate Dehydrogenase Internal medicine medicine Animals Bile Propyl Gallate Chromans Rats Wistar Propyl gallate Pharmacology L-Lactate Dehydrogenase Chemistry Glutathione Ascorbic acid Cell Hypoxia Rats Disease Models Animal Endocrinology Liver Trolox Oxidative stress |
| Zdroj: | Pharmacology & Toxicology. 71:278-283 |
| ISSN: | 1600-0773 0901-9928 |
| DOI: | 10.1111/j.1600-0773.1992.tb00983.x |
| Popis: | Isolated perfused livers from rats fasted overnight were subjected to 30 min. of hypoxia followed by reoxygenation for 60 min., resulting in marked cytotoxicity as evidenced by an enhanced release of cytosolic enzymes (lactate dehydrogen-ase: 14-fold over controls, glutamate-pyruvate-transaminase: 12-fold over controls) and glutathione (twofold over controls) into the perfusate, by calcium accumulation (by a Factor of 1.4) in the tissue and by an 80% inhibition of bile secretion. Virtually no mitochondria1 injury became apparent and no evidence for lipid peroxidation could be found. In the presence of ascorbate, an augmentation of hepatic injury was observed. This might be due to the pro-oxidant activity of ascorbate in the presence of ionized iron, which is easily released from high molecular weight stores under reductive (e.g. hypoxic) conditions. The water soluble vitamin E analogue trolox C as well as propyl gallate clearly protected the liver against hypoxiaireoxygenation injury, yielding further evidence for a causative role of oxidative stress in this model. Due to their water solubility and their high efficacy as free radical scavengers, these antioxidants might be of therapeutic value. |
| Databáze: | OpenAIRE |
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