Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking
| Autor: | Francisco Blanco-Favela, Adriana Karina Chávez-Rueda, Rocio Flores-Fernández, Patricia Gorocica-Rosete, Luis Chávez-Sánchez, Alberto Pizaña-Venegas, Ezequiel M. Fuentes-Pananá |
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| Rok vydání: | 2016 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
0301 basic medicine Mice Inbred MRL lpr endocrine system Programmed cell death medicine.medical_specialty Article Subject Cell Survival Receptors Prolactin Immunology B-cell receptor Gene Expression Receptors Antigen B-Cell Apoptosis Mice 03 medical and health sciences Antigen immune system diseases Annexin Cell Line Tumor Internal medicine medicine Animals Immunology and Allergy skin and connective tissue diseases Receptor Systemic lupus erythematosus Caspase 3 business.industry Precursor Cells B-Lymphoid General Medicine medicine.disease Prolactin 030104 developmental biology Endocrinology Cell culture lcsh:RC581-607 business hormones hormone substitutes and hormone antagonists Research Article Protein Binding |
| Zdroj: | Journal of Immunology Research, Vol 2016 (2016) Journal of Immunology Research |
| ISSN: | 2314-7156 2314-8861 |
| Popis: | Prolactin has an immunomodulatory effect and has been associated with B-cell-triggered autoimmune diseases, such as systemic lupus erythematosus (SLE). In mice that develop SLE, the PRL receptor is expressed in early bone marrow B-cells, and increased levels of PRL hasten disease manifestations, which are correlated with a reduction in the absolute number of immature B-cells. The aim of this work was to determine the effect of PRL in anin vitrosystem of B-cell tolerance using WEHI-231 cells and immature B-cells from lupus prone MRL/lpr mice. WEHI-231 cells express the long isoform of the PRL receptor, and PRL rescued the cells from cell death by decreasing the apoptosis induced by the cross-linking of the B-cell antigen receptor (BCR) as measured by Annexin V and active caspase-3. This decrease in apoptosis may have been due to the PRL and receptor interaction, which increased the relative expression of antiapoptotic Bcl-xL and decreased the relative expression of proapoptotic Bad. In immature B-cells from MRL/lpr mice, PRL increased the viability and decreased the apoptosis induced by the cross-linking of BCR, which may favor the maturation of self-reactive B-cells and contribute to the onset of disease. |
| Databáze: | OpenAIRE |
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