High‐throughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistantStaphylococcus aureusand underlines a tailored response to different osmotic stressors
Autor: | Schuster, Christopher F., Wiedemann, David M., Kirsebom, Freja C. M., Santiago, Marina, Walker, Suzanne, Gründling, Angelika |
---|---|
Přispěvatelé: | Wellcome Trust, Medical Research Council (MRC) |
Rok vydání: | 2019 |
Předmět: |
Penicillin binding proteins
Mutant medicine.disease_cause Bacterial cell structure KCl chemistry.chemical_compound Osmoregulation NaCl Cell Wall Proline transport AMINO-ACIDS 11 Medical and Health Sciences Research Articles 0303 health sciences DUF25380 High-Throughput Nucleotide Sequencing sucrose PBP2 Staphylococcal Infections CAPSULAR POLYSACCHARIDE Staphylococcus aureus TEICHOIC-ACIDS GROWTH Life Sciences & Biomedicine Research Article Methicillin-Resistant Staphylococcus aureus Biochemistry & Molecular Biology Osmotic shock Peptidoglycan Biology Staphylococcal infections aureus GLYCINE BETAINE Microbiology Cell wall 03 medical and health sciences C-DI-AMP Osmotic Pressure 07 Agricultural and Veterinary Sciences medicine Humans Penicillin-Binding Proteins Molecular Biology 030304 developmental biology Science & Technology 030306 microbiology 06 Biological Sciences S. aureus medicine.disease chemistry PROLINE TRANSPORT DNA Transposable Elements osmotic stress |
Zdroj: | Molecular Microbiology |
ISSN: | 1365-2958 0950-382X |
DOI: | 10.1111/mmi.14433 |
Popis: | Staphylococcus aureus is an opportunistic pathogen that can cause soft tissue infections but is also a frequent cause of foodborne illnesses. One contributing factor for this food association is its high salt tolerance allowing this organism to survive commonly used food preservation methods. How this resistance is mediated is poorly understood, particularly during long‐term exposure. In this study, we used transposon sequencing (TN‐seq) to understand how the responses to osmotic stressors differ. Our results revealed distinctly different long‐term responses to NaCl, KCl and sucrose stresses. In addition, we identified the DUF2538 domain containing gene SAUSA300_0957 (gene 957) as essential under salt stress. Interestingly, a 957 mutant was less susceptible to oxacillin and showed increased peptidoglycan crosslinking. The salt sensitivity phenotype could be suppressed by amino acid substitutions in the transglycosylase domain of the penicillin‐binding protein Pbp2, and these changes restored the peptidoglycan crosslinking to WT levels. These results indicate that increased crosslinking of the peptidoglycan polymer can be detrimental and highlight a critical role of the bacterial cell wall for osmotic stress resistance. This study will serve as a starting point for future research on osmotic stress response and help develop better strategies to tackle foodborne staphylococcal infections. Staphylococcus aureus is able to grow in the presence of high concentrations of NaCl but the exact genetic factors contributing to this are unknown. Using a high‐throughput TN‐seq approach, we identified gene 957 as an important factor for the salt stress resistance in S. aureus. A 957‐mutant was not only salt sensitive but also showed increased peptidoglycan crosslinking, altogether highlighting the cell wall as an important barrier against osmotic stress. |
Databáze: | OpenAIRE |
Externí odkaz: |