High‐throughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistantStaphylococcus aureusand underlines a tailored response to different osmotic stressors

Autor: Schuster, Christopher F., Wiedemann, David M., Kirsebom, Freja C. M., Santiago, Marina, Walker, Suzanne, Gründling, Angelika
Přispěvatelé: Wellcome Trust, Medical Research Council (MRC)
Rok vydání: 2019
Předmět:
Penicillin binding proteins
Mutant
medicine.disease_cause
Bacterial cell structure
KCl
chemistry.chemical_compound
Osmoregulation
NaCl
Cell Wall
Proline transport
AMINO-ACIDS
11 Medical and Health Sciences
Research Articles
0303 health sciences
DUF25380
High-Throughput Nucleotide Sequencing
sucrose
PBP2
Staphylococcal Infections
CAPSULAR POLYSACCHARIDE
Staphylococcus aureus
TEICHOIC-ACIDS
GROWTH
Life Sciences & Biomedicine
Research Article
Methicillin-Resistant Staphylococcus aureus
Biochemistry & Molecular Biology
Osmotic shock
Peptidoglycan
Biology
Staphylococcal infections
aureus
GLYCINE BETAINE
Microbiology
Cell wall
03 medical and health sciences
C-DI-AMP
Osmotic Pressure
07 Agricultural and Veterinary Sciences
medicine
Humans
Penicillin-Binding Proteins
Molecular Biology
030304 developmental biology
Science & Technology
030306 microbiology
06 Biological Sciences
S. aureus
medicine.disease
chemistry
PROLINE TRANSPORT
DNA Transposable Elements
osmotic stress
Zdroj: Molecular Microbiology
ISSN: 1365-2958
0950-382X
DOI: 10.1111/mmi.14433
Popis: Staphylococcus aureus is an opportunistic pathogen that can cause soft tissue infections but is also a frequent cause of foodborne illnesses. One contributing factor for this food association is its high salt tolerance allowing this organism to survive commonly used food preservation methods. How this resistance is mediated is poorly understood, particularly during long‐term exposure. In this study, we used transposon sequencing (TN‐seq) to understand how the responses to osmotic stressors differ. Our results revealed distinctly different long‐term responses to NaCl, KCl and sucrose stresses. In addition, we identified the DUF2538 domain containing gene SAUSA300_0957 (gene 957) as essential under salt stress. Interestingly, a 957 mutant was less susceptible to oxacillin and showed increased peptidoglycan crosslinking. The salt sensitivity phenotype could be suppressed by amino acid substitutions in the transglycosylase domain of the penicillin‐binding protein Pbp2, and these changes restored the peptidoglycan crosslinking to WT levels. These results indicate that increased crosslinking of the peptidoglycan polymer can be detrimental and highlight a critical role of the bacterial cell wall for osmotic stress resistance. This study will serve as a starting point for future research on osmotic stress response and help develop better strategies to tackle foodborne staphylococcal infections.
Staphylococcus aureus is able to grow in the presence of high concentrations of NaCl but the exact genetic factors contributing to this are unknown. Using a high‐throughput TN‐seq approach, we identified gene 957 as an important factor for the salt stress resistance in S. aureus. A 957‐mutant was not only salt sensitive but also showed increased peptidoglycan crosslinking, altogether highlighting the cell wall as an important barrier against osmotic stress.
Databáze: OpenAIRE