CD14 expression by human mononuclear phagocytes is modulated by Clostridium difficile toxin B
| Autor: | Phillipe Poindron, Dumont S, Christian D. Muller, Jean-Claude Siffert, Henri Monteil |
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| Rok vydání: | 1999 |
| Předmět: |
Time Factors
Toxin Macrophages Monocyte CD14 Bacterial Toxins Immunology Lipopolysaccharide Receptors Clostridium difficile toxin A Clostridium difficile toxin B Pseudomembranous colitis Biology Flow Cytometry medicine.disease_cause Microbiology Monocytes Infectious Diseases medicine.anatomical_structure Bacterial Proteins medicine Humans Macrophage Cells Cultured Exotoxin |
| Zdroj: | Microbes and Infection. 1:1159-1162 |
| ISSN: | 1286-4579 |
| DOI: | 10.1016/s1286-4579(99)00247-6 |
| Popis: | Toxin B, an exotoxin produced by the anaerobic Gram-positive bacteria Clostridium difficile, is responsible for pseudomembranous colitis in humans. It deeply modifies morphology of cultured cells and enhances their membrane surface area, which suggests a possible alteration of membrane receptor distribution. Since toxin B and bacterial lipopolysaccharide can act synergistically on TNF-alpha production by mononuclear phagocytes, the effect of toxin B on CD14 expression was investigated using flow cytometric analysis. It was shown that monocytes overexpressed CD14 after 5 h of treatment with toxin B. In contrast, after 24 h of treatment, the percentage of CD14 monocytes decreased, although, most frequently, the remaining positive cells expressed high levels of CD14 compared with untreated cells. Macrophages treated for 5 h with toxin B overexpressed CD14, but this effect persisted for at least 24 h. Both the percentage of positive macrophages and the mean level of CD14 per cell were increased. Thus toxin B can modulate expression of CD14 and its modulation depends on the differentiation status and maybe on the activation state, since some individual variations were observed in monocyte response to toxin. |
| Databáze: | OpenAIRE |
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