Macrophage Accumulation and Angiogenesis in Epicardial Adipose Tissue in Cardiac Patients with or without Chronic Heart Failure

Autor: Radu Danila, Doina Butcovan, R. Haliga, Bogdan Mihnea Ciuntu, Radu Sascau, Gabriela Ghiga, Veronica Mocanu, Cristian Stătescu, Victor Vlad Costan, Daniel Timofte, Adina Pricope Veselin
Rok vydání: 2020
Předmět:
medicine.medical_specialty
Angiogenesis
Inflammation
030204 cardiovascular system & hematology
lcsh:Technology
right atrial appendages
lcsh:Chemistry
Coronary artery disease
03 medical and health sciences
0302 clinical medicine
coronary artery bypass graft
Internal medicine
medicine
General Materials Science
cardiovascular diseases
030212 general & internal medicine
lcsh:QH301-705.5
Instrumentation
Fluid Flow and Transfer Processes
Ejection fraction
lcsh:T
CD68
business.industry
Process Chemistry and Technology
General Engineering
epicardial adipose tissue
medicine.disease
lcsh:QC1-999
chronic heart failure
Computer Science Applications
medicine.anatomical_structure
lcsh:Biology (General)
lcsh:QD1-999
lcsh:TA1-2040
Heart failure
cardiovascular system
Cardiology
Immunohistochemistry
medicine.symptom
lcsh:Engineering (General). Civil engineering (General)
business
lcsh:Physics
coronary artery disease
Artery
Zdroj: Applied Sciences
Volume 10
Issue 17
Applied Sciences, Vol 10, Iss 5871, p 5871 (2020)
ISSN: 2076-3417
DOI: 10.3390/app10175871
Popis: Routinely measuring epicardial fat had become a novel tool for cardiovascular risk stratification. Structural changes in epicardial adipose tissue (EAT), including fat thickness, inflammation, and angiogenesis, have been described in coronary artery disease (CAD) patients. We proposed to measure EAT thickness and characterize inflammatory infiltrate and angiogenesis in epicardial adipose tissue in CAD patients with and without chronic heart failure (CHF), established by cardiac dysfunction on echocardiography (left ventricular ejection fraction, LVEF &le
50%) and symptoms of heart failure (New York Heart Association (NYHA) functional class II or III).The study included 15 patients with CAD (demonstrated by coronary angiography),, who underwent right atrial appendages (RAA) excision during coronary artery bypass graft (CABG). The study was performed by histopathological, immunohistochemical (IHC), and morphometrical analysis. EAT thickness was assessed by using morphometry applied on routine histological stains. Inflammatory cell infiltration and angiogenesis were investigated immunohistochemically by using antibodies against CD68 and CD34 markers. Diminished EAT thickness in the CAD patients with CHF was associated with increased macrophage infiltration and reduced angiogenesis of the EAT as compared to CAD patients without CHF. In conclusion, the present study on epicardial fat samples of the RAA suggested that high expression of CD68 appeared to be associated with severe deterioration of heart function in CAD patients who underwent myocardial revascularization consisting of CABG.
Databáze: OpenAIRE
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