Antihyperglycemic and antioxidative effect of hydro - methanolic (2:3) extract of the seed of Swietenia mahagoni (L.) Jacq. in streptozotocin-induced diabetic male albino rat: An approach through pancreas
| Autor: | Debidas Ghosh, Kazi Monjur Ali, Debasis De, Soumyajit Maiti, Tushar Kanti Bera, Kausik Chatterjee, Kishalay Jana |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Thiobarbituric acid medicine.medical_treatment Biomedical Engineering General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Carbohydrate metabolic enzymes Internal medicine Diabetes mellitus Drug Discovery medicine TBARS Swietenia mahagoni Insulin Pancreas Diminution business.industry Pancreatic islets General Medicine Streptozotocin medicine.disease Metformin Endocrinology medicine.anatomical_structure chemistry business medicine.drug |
| Zdroj: | Genomic Medicine, Biomarkers, and Health Sciences. 4(4):107-117 |
| ISSN: | 2211-4254 |
| DOI: | 10.1016/j.gmbhs.2012.11.001 |
| Popis: | The goal of this study was to investigate the effects of the hydro - methanolic (2:3) extract of the seed of Swietenia mahagoni (L.) Jacq. for the management of streptozotocin (STZ)-induced diabetes. Wistar rats were divided equally into four groups (n = 6): normal control, diabetes control, diabetes + extract treated, and diabetes + metformin treated. The extract (25 mg/100 g by weight) and metformin (2.5 mg/100 g by weight) were administered once a day, orally by gavages for 21 days at fasting condition. Hexokinase, glucose-6-phosphate dehydrogenase, and glucose-6-phosphatase activities were measured in hepatic tissue. Activities of catalase (CAT), peroxidase (Px), and superoxide dismutase (SOD), along with the quantity of thiobarbituric acid reactive substances (TBARS) and conjugated diene (CD) in hepatic and renal tissues, were assessed. Histoarchitecture of the pancreas and serum insulin level were also evaluated. Significant diminution in the activities of hepatic hexokinase and glucose-6-phosphate dehydrogenase along with elevation in glucose-6-phosphatase were noted in STZ-induced animals with diabetes in respect to control animals. Level of fasting blood glucose (FBG) was elevated in animals with diabetes. Activities of CAT, Px, and SOD were diminished significantly along with the elevation in TBARS and CD levels in animals with diabetes. Diameter of pancreatic islets, count of islets, and degeneration in pancreatic acini were also noted in animals with diabetes. Treatment of these animals with extract or metformin resulted in substantial recovery in the aforementioned biosensors toward the control level. This recovery is not equal to metformin because the plant extract is not the pure form of effective ingredient(s) but provides insight to the pharmaceutical industries that the extract has a protective therapeutic effect against diabetes through s-cell regeneration capacity. |
| Databáze: | OpenAIRE |
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