Inhibition of platelet-activating factor- and zymosan-activated serum-induced chemotaxis of human neutrophils by nedocromil sodium, BN 52021 and sodium cromoglycate
| Autor: | R.A.J. Warringa, P.T.M. Kok, P.L.B. Bruijnzeel |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Nedocromil
Neutrophils Neutrophile In Vitro Techniques Quinolones Pharmacology Lactones chemistry.chemical_compound Cromolyn Sodium medicine Humans Platelet Activating Factor Nedocromil Sodium IC50 Platelet-activating factor Zymosan Chemotaxis respiratory system Chemotaxis Leukocyte Ginkgolides chemistry Immunology Calcium Diterpenes Research Article Granulocytes medicine.drug |
| Zdroj: | British Journal of Pharmacology. 97:1251-1257 |
| ISSN: | 0007-1188 |
| DOI: | 10.1111/j.1476-5381.1989.tb12586.x |
| Popis: | 1. Inflammatory cells such as eosinophils and neutrophils are thought to contribute actively to the pathogenesis of asthma since they infiltrate into the lung tissue. These cells are mobilized by lipid-like and protein-like chemotactic factors. As illustrative examples of both groups, platelet-activating-factor (Paf) and zymosan-activated-serum (ZAS) were used in this study. The inhibitory effects of nedocromil sodium, the Paf antagonist BN 52021 and sodium cromoglycate on Paf- and ZAS-induced neutrophil chemotaxis were evaluated. 2. All tested drugs inhibited Paf-induced neutrophil chemotaxis with approximately the same potency (IC50 approximately 1 nM). 3. Nedocromil sodium and sodium cromoglycate were equally potent in inhibiting ZAS-induced neutrophil chemotaxis (IC50 = 0.1-1 microM), whereas BN 52021 was considerably less potent (IC30 = 10 microM). 4. To find out whether the drugs tested could inhibit early events in cell activation, their capacity to inhibit Paf- and ZAS-induced cytosolic free Ca2+-mobilization was investigated. BN 52021, at a concentration of 100 microM, completely inhibited Paf-induced Ca2+-mobilization and inhibited ZAS-induced Ca2+-mobilization by about 50%. Nedocromil sodium and sodium cromoglycate were ineffective. |
| Databáze: | OpenAIRE |
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