Surgical experimental jejunoileal atresia in rat embryo
| Autor: | Guénolée de Lambert, Anais Victor, Laurent Fourcade, Nadine Cerf-Bensussan, Sabine Sarnacki, Frédérique Sauvat, Naziha Khen-Dunlop |
|---|---|
| Přispěvatelé: | CHU Necker - Enfants Malades [AP-HP], Neuropathies du système nerveux entérique et pathologies digestives, implication des cellules gliales entériques, Université de Nantes ( UN ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Biomolécules Thérapies anti-tumorales ( EA4021 ), Université de Limoges ( UNILIM ) -Génomique, Environnement, Immunité, Santé, Thérapeutique ( GEIST FR CNRS 3503 ), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biomolécules Thérapies anti-tumorales (EA4021), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503) |
| Rok vydání: | 2009 |
| Předmět: |
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
medicine.medical_treatment [ SDV.MHEP.PED ] Life Sciences [q-bio]/Human health and pathology/Pediatrics Abdominal cavity 030230 surgery Gastroenterology MESH : Intestinal Atresia MESH: Pregnancy 0302 clinical medicine Pregnancy MESH: Gestational Age MESH: Animals MESH : Female Hysterotomy MESH : Jejunum [ SDV.MHEP.CHI ] Life Sciences [q-bio]/Human health and pathology/Surgery MESH : Ligation [ SDV.GEN.GH ] Life Sciences [q-bio]/Genetics/Human genetics MESH : Rats Intestinal atresia Gestational age General Medicine 3. Good health Fetal Diseases MESH: Fetal Diseases Jejunum medicine.anatomical_structure [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [ SDV.NEU.NB ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Female medicine.medical_specialty MESH: Rats Intestinal Atresia Gestational Age [SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery [ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology 03 medical and health sciences Ileum [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] 030225 pediatrics Internal medicine medicine Animals Ligation Survival rate MESH : Ileum MESH: Intestinal Atresia [SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics Fetus business.industry Fetal surgery MESH : Fetal Diseases [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology MESH: Ligation medicine.disease MESH : Disease Models Animal Rats Surgery MESH : Pregnancy Disease Models Animal [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics MESH: Jejunum MESH: Ileum [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Atresia Pediatrics Perinatology and Child Health MESH : Animals MESH: Disease Models Animal business MESH: Female MESH : Gestational Age |
| Zdroj: | Journal of Pediatric Surgery Journal of Pediatric Surgery, American Academy of Pediatrics, 2009, 44 (9), pp.1725-9. 〈10.1016/j.jpedsurg.2008.12.017〉 Journal of Pediatric Surgery, American Academy of Pediatrics, 2009, 44 (9), pp.1725-9. ⟨10.1016/j.jpedsurg.2008.12.017⟩ |
| ISSN: | 0022-3468 1531-5037 |
| DOI: | 10.1016/j.jpedsurg.2008.12.017 |
| Popis: | International audience; PURPOSE: Jejunoileal atresia represents about 40% of intestinal atresia. After surgical repair, intestinal motility disorders are burdened with the postoperative outcome, and the origin of these troubles remains unclear. To specify the physiopathologic feature of jejunoileal atresia, we developed an experimental surgical model in fetal rat. METHODS: Time-dated pregnant rats were operated on at 18 days of gestational age. Hysterotomy was performed, followed by fetal wall incision. The exteriorization of the bowel loop was obtained by saline injection; the intestine was ligated and returned to the abdominal cavity before incisions were closed. Fetal intestine was excised at day 21, after cesarean delivery. RESULTS: Twenty-one pregnant rats underwent operation with 90% maternal survival rate. Among the 56 fetuses successfully operated on, 49 survived (87%). In fetuses with atresia, the mean birth weight (4.5 +/- 0.6 g) and the mean intestinal length (12.8 +/- 1.3 cm) were significantly lower compared to sham fetuses and controls. CONCLUSION: The rat model offers the advantage of a low-expense mammal model with a wide panel of probes and reagents available for the study of the gut. This model of jejunoileal atresia could be used to study the consequences of prenatal intestinal obstruction on fetal gut. |
| Databáze: | OpenAIRE |
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