Magnetic Resonance Imaging of Stem Cell Apoptosis in Arthritic Joints with a Caspase Activatable Contrast Agent
| Autor: | Barbara Sennino, Jianghong Rao, Olga Lenkov, Deju Ye, Jessica Donig, Nikita Derugin, John E. Martin, Rostislav Castillo, Heike E. Daldrup-Link, Hossein Nejadnik |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Materials science Cell Contrast Media General Physics and Astronomy Arthritis Apoptosis Caspase 3 Osteoarthritis Article medicine Animals General Materials Science medicine.diagnostic_test Hydrolysis Stem Cells Regeneration (biology) Cartilage General Engineering Magnetic resonance imaging Anatomy medicine.disease Magnetic Resonance Imaging Rats medicine.anatomical_structure Nanoparticles Female Joints Stem cell Stem Cell Transplantation |
| Zdroj: | ACS Nano. 9:1150-1160 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/nn504494c |
| Popis: | About 43 million individuals in the U.S. encounter cartilage injuries due to trauma or osteoarthritis, leading to joint pain and functional disability. Matrix-associated stem cell implants (MASI) represent a promising approach for repair of cartilage defects. However, limited survival of MASI creates a significant bottleneck for successful cartilage regeneration outcomes and functional reconstitution. We report an approach for noninvasive detection of stem cell apoptosis with magnetic resonance imaging (MRI), based on a caspase-3-sensitive nanoaggregation MRI probe (C-SNAM). C-SNAM self-assembles into nanoparticles after hydrolysis by caspase-3, leading to 90% amplification of (1)H MR signal and prolonged in vivo retention. Following intra-articular injection, C-SNAM causes significant MR signal enhancement in apoptotic MASI compared to viable MASI. Our results indicate that C-SNAM functions as an imaging probe for stem cell apoptosis in MASI. This concept could be applied to a broad range of cell transplants and target sites. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|