Hexose Transporter Expression and Function in Mouse Small Intestine: Role of Diurnal Rhythm
| Autor: | Ye Zheng, Javairiah Fatima, Michael G. Sarr, Corey W. Iqbal, Judith A. Duenes, Scott G. Houghton, Michael S. Kasparek |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Monosaccharide Transport Proteins Duodenum Glucose Transport Proteins Facilitative Ileum Biology Article Jejunum Mice Sodium-Glucose Transporter 1 Intestinal mucosa Internal medicine Intestine Small medicine Animals Hexose RNA Messenger Circadian rhythm Intestinal Mucosa Glucose Transporter Type 2 chemistry.chemical_classification Glucose Transporter Type 5 Gastroenterology Transporter Small intestine Circadian Rhythm Mice Inbred C57BL medicine.anatomical_structure Endocrinology chemistry Surgery |
| Zdroj: | Journal of Gastrointestinal Surgery. 13:634-641 |
| ISSN: | 1873-4626 1091-255X |
| Popis: | Expression and function of hexose transporters vary diurnally in rat small intestine; however, this subject remains unexplored in mice.The aim of the study was to investigate the diurnal expression and function of hexose transporters SGLT1, GLUT2, and GLUT5 in mouse small bowel.Twenty-four c57bl6 mice maintained in a 12-h light/dark room (6 AM: -6 PM: ) were sacrificed at 9 AM: , 3 PM: , 9 PM: , and 3 AM: (n = 6 each). In duodenal, jejunal, and ileal mucosa, total cellular mRNA and protein levels were quantitated by real-time PCR and semiquantitative Western blotting, respectively. The everted sleeve technique measured transporter-mediated glucose uptake at 9 AM: and 9 PM: .mRNA expression of SGLT1, GLUT2, and GLUT5 varied diurnally in all three intestinal segments (por= 0.03). SGLT1, GLUT2, and GLUT5 protein levels varied diurnally in duodenum and jejunum (p0.05) but not in ileum. Transporter-mediated glucose uptake was greater at 9 PM: than 9 AM: (por= 0.04) in all three segments. V (max) was greater in duodenum (10 vs 6 nmol/cm/s) and jejunum (8 vs 5 nmol/cm/s) at 9 PM: compared to 9 AM: (p = 0.01); K (m) remained unchanged. mRNA levels of intestinal hexose transporters varied diurnally. Protein levels peaked 6-12 h later during dark cycle when70% of food intake occurred; glucose transport followed a similar pattern with increased uptake at 9 PM: .Hexose transporter expression and function vary diurnally with nocturnal feeding patterns of mice. |
| Databáze: | OpenAIRE |
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