Proteomic analysis of p16ink4a-binding proteins
| Autor: | E Olmedo, Núria Canela, Elielson Souza-Rodrígues, Neus Agell, Erica Friedrich-Heineken, Jorge Vera, Sonia Brun, Montserrat Jaumot, Ulrich Hübscher, Oriol Bachs, Josep Maria Estanyol |
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| Rok vydání: | 2007 |
| Předmět: |
Proteomics
Immunoprecipitation Blotting Western Fluorescent Antibody Technique Biology Biochemistry DNA-binding protein DNA polymerase delta Sensitivity and Specificity Chromatography Affinity Mice Minichromosome maintenance Cell Line Tumor Proliferating Cell Nuclear Antigen Tumor Cells Cultured Animals Humans neoplasms Molecular Biology Polymerase Cyclin-Dependent Kinase Inhibitor p16 DNA Polymerase III integumentary system Binding protein Molecular biology Cell biology Proliferating cell nuclear antigen Enzyme Activation Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization embryonic structures biology.protein Electrophoresis Polyacrylamide Gel biological phenomena cell phenomena and immunity HeLa Cells |
| Zdroj: | Proteomics. 7(22) |
| ISSN: | 1615-9853 |
| Popis: | The p16(ink4a) tumor suppressor protein plays a critical role in cell cycle control, tumorogenesis and senescence. The best known activity for p16(ink4a) is the inhibition of the activity of CDK4 and CDK6 kinases, both playing a key role in cell cycle progression. With the aim to study new p16(ink4a) functions we used affinity chromatography and MS techniques to identify new p16(ink4a)-interacting proteins. We generated p16(ink4a) columns by coupling the protein to activated Sepharose 4B. The proteins from MOLT-4 cell line that bind to p16(ink4a) affinity columns were resolved by SDS-PAGE and identified by MS using a MALDI-TOF. Thirty-one p16(ink4a) -interacting proteins were identified and grouped in functional clusters. The identification of two of them, proliferating cell nuclear antigen (PCNA) and minichromosome maintenance protein 6 (MCM6), was confirmed by Western blotting and their in vivo interactions with p16(ink4a) were demonstrated by immunoprecipitation and immunofluorescence studies. Results also revealed that p16(ink4a) interacts directly with the DNA polymerase delta accessory protein PCNA and thereby inhibits the polymerase activity. |
| Databáze: | OpenAIRE |
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