A hybrid P3HT-Graphene interface for efficient photostimulation of neurons
| Autor: | Guglielmo Lanzani, Fabio Benfenati, Elisabetta Colombo, Giovanni Manfredi, Mattia L. DiFrancesco, Ermanno D. Papaleo, José Fernando Maya-Vetencourt |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Organic solar cell Biocompatibility Retinal implant FOS: Physical sciences Nanotechnology Context (language use) Biointerface 02 engineering and technology 010402 general chemistry 01 natural sciences law.invention Photostimulation PEDOT:PSS law Cell Behavior (q-bio.CB) General Materials Science Physics - Biological Physics Graphene General Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences 3. Good health Biological Physics (physics.bio-ph) Quantitative Biology - Neurons and Cognition FOS: Biological sciences Quantitative Biology - Cell Behavior Neurons and Cognition (q-bio.NC) 0210 nano-technology |
| Zdroj: | Carbon |
| ISSN: | 0008-6223 |
| Popis: | Graphene conductive properties have been long exploited in the field of organic photovoltaics and optoelectronics by the scientific community worldwide. We engineered and characterized a hybrid biointerface in which graphene is coupled with photosensitive polymers, and tested its ability to elicit lighttriggered neural activity modulation in primary neurons and blind retina explants. We designed such a graphene-based device by modifying a photoactive P3HT-based retinal interface, previously reported to rescue light sensitivity in blind rodents, with a CVD graphene layer replacing the conductive PEDOT:PSS layer to enhance charge separation. The new graphene-based device was characterized for its electrochemical features and for the ability to photostimulate primary neurons and blind retina explants, while preserving biocompatibility. Light-triggered responses, recorded by patch-clamp in vitro or MEA ex vivo, show a stronger light-transduction efficiency when the neurons are interfaced with the graphene-based device with respect to the PEDOT:PSS-based one. The possibility to ameliorate flexible photo-stimulating devices via the insertion of graphene, paves the way for potential biomedical applications of graphenebased neuronal interfaces in the context of retinal implants. Comment: 10 pages, 5 figures |
| Databáze: | OpenAIRE |
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