Epo Is Relevant Neither for Microvascular Formation Nor for the New Formation and Maintenance of Mice Skeletal Muscle Fibres in Both Normoxia and Hypoxia

Autor: Luciana Hagström, Thierry Launay, Raja El-Hasnaoui-Saadani, Onnik Agbulut, Jean-Paul Richalet, Dominique Marchant, Fabrice Favret, Michèle Beaudry
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Male
Sarcomeres
medicine.medical_specialty
Article Subject
Health
Toxicology and Mutagenesis
medicine.medical_treatment
lcsh:Biotechnology
Muscle Fibers
Skeletal
Neovascularization
Physiologic
lcsh:Medicine
Mice
Transgenic
Biology
Sarcomere
Statistics
Nonparametric
Mice
Downregulation and upregulation
Internal medicine
hemic and lymphatic diseases
lcsh:TP248.13-248.65
Genetics
medicine
Receptors
Erythropoietin
Animals
Hypoxia
Muscle
Skeletal
Molecular Biology
Erythropoietin
Analysis of Variance
Histocytochemistry
Vascular Endothelial Growth Factors
Growth factor
lcsh:R
Oxygen transport
Skeletal muscle
General Medicine
Hypoxia (medical)
Erythropoietin receptor
Up-Regulation
Muscular Atrophy
medicine.anatomical_structure
Endocrinology
Microvessels
Molecular Medicine
Hypoxia-Inducible Factor 1
medicine.symptom
Biotechnology
medicine.drug
Research Article
Zdroj: Journal of Biomedicine and Biotechnology, Vol 2010 (2010)
Journal of Biomedicine and Biotechnology
ISSN: 1110-7251
1110-7243
Popis: Erythropoietin (Epo) and vascular growth factor (VEGF) are known to be involved in the regulation of cellular activity when oxygen transport is reduced as in anaemia or hypoxic conditions. Because it has been suggested that Epo could play a role in skeletal muscle development, regeneration, and angiogenesis, we aimed to assess Epo deficiency in both normoxia and hypoxia by using an Epo-deficient transgenic mouse model ( E p o - T A g h ). Histoimmunology, ELISA and real time RT-PCR did not show any muscle fiber atrophy or accumulation of active HIF-1 𝛼 but an improvement of microvessel network and an upregulation of VEGFR2 mRNA in Epo-deficient gastrocnemius compared with Wild-Type one. In hypoxia, both models exhibit an upregulation of VEGF120 and VEGFR2 mRNA but no accumulation of Epo protein. EpoR mRNA is not up-regulated in both Epo-deficient and hypoxic gastrocnemius. These results suggest that muscle deconditioning observed in patients suffering from renal failure is not due to Epo deficiency.
Databáze: OpenAIRE
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