Extracellular calcium triggers unique transcriptional programs and modulates staurosporine-induced cell death in Neurospora crassa
| Autor: | Daniel J. Rigden, N. L. Glass, António Pedro Gonçalves, Paulo Correia-de-Sá, J. M. Cordeiro, João Monteiro, Chiara Lucchi, Arnaldo Videira, David Kowbel |
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| Rok vydání: | 2014 |
| Předmět: |
Programmed cell death
1.1 Normal biological development and functioning Applied Microbiology chemistry.chemical_element Calcium Biochemistry Genetics and Molecular Biology (miscellaneous) Microbiology Applied Microbiology and Biotechnology Neurospora crassa 03 medical and health sciences Underpinning research Virology Genetics Extracellular medicine Staurosporine lcsh:QH301-705.5 Molecular Biology Ca2+-binding motif 030304 developmental biology chemistry.chemical_classification 0303 health sciences Reactive oxygen species calcium biology 030306 microbiology ROS Cell Biology biology.organism_classification Cell biology Cytosol cell death lcsh:Biology (General) chemistry Parasitology Intracellular medicine.drug |
| Zdroj: | Microbial Cell Microbial Cell, Vol 1, Iss 9, Pp 289-302 (2014) Gonçalves, AP; Monteiro, J; Lucchi, C; Kowbel, DJ; Cordeiro, JM; Correia-de-Sá, P; et al.(2014). Extracellular calcium triggers unique transcriptional programs and modulates staurosporine-induced cell death in Neurospora crassa.. Microbial cell (Graz, Austria), 1(9), 289-302. doi: 10.15698/mic2014.09.165. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/5r85s0c7 Microbial cell (Graz, Austria), vol 1, iss 9 |
| ISSN: | 2311-2638 |
| DOI: | 10.15698/mic2014.09.165 |
| Popis: | Alterations in the intracellular levels of calcium are a common response to cell death stimuli in animals and fungi and, particularly, in the Neurospora crassa response to staurosporine. We highlight the importance of the extracellular availability of Ca2+ for this response. Limitation of the ion in the culture medium further sensitizes cells to the drug and results in increased accumulation of reactive oxygen species (ROS). Conversely, an approximately 30-fold excess of external Ca2+ leads to increased drug tolerance and lower ROS generation. In line with this, distinct staurosporine-induced cytosolic Ca2+ signaling profiles were observed in the absence or presence of excessive external Ca2+. High-throughput RNA sequencing revealed that different concentrations of extracellular Ca2+ define distinct transcriptional programs. Our transcriptional profiling also pointed to two putative novel Ca2+-binding proteins, encoded by the NCU08524 and NCU06607 genes, and provides a reference dataset for future investigations on the role of Ca2+ in fungal biology. |
| Databáze: | OpenAIRE |
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