Disinfection Efficacy of Current Regenerative Endodontic Protocols in Simulated Necrotic Immature Permanent Teeth
| Autor: | Anahid Jewett, Jason Latham, Hanson Fong, James D. Johnson, Avina Paranjpe |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Regenerative endodontics Materials science medicine.drug_class medicine.medical_treatment Antibiotics Dentistry Minocycline In Vitro Techniques Enterococcus faecalis Calcium Hydroxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ciprofloxacin Metronidazole medicine Dental Pulp Necrosis Humans General Dentistry Permanent teeth Debridement Calcium hydroxide Microscopy Confocal biology business.industry 030206 dentistry biology.organism_classification Anti-Bacterial Agents Dentition Permanent Disinfection Incisor Drug Combinations 030104 developmental biology chemistry Microscopy Electron Scanning Dental Pulp Cavity business medicine.drug |
| Zdroj: | Journal of endodontics. 42(8) |
| ISSN: | 1878-3554 |
| Popis: | Introduction The lack of mechanical debridement and reduced concentrations suggested for chemical debridement to maintain stem cell viability call into question the disinfection efficacy of current regenerative protocols. Current protocols vary in the concentration and type of antibiotic medicaments used. The aim of this study was to determine if simulated immature teeth infected with Enterococcus faecalis can be completely disinfected by following current standardized regenerative protocols and to evaluate the probable effects of residual bacteria on stem cell toxicity. Methods Sixty-eight caries-free maxillary incisors were used. S1 sampling protocols were validated in both negative and positive control groups via culture, scanning electron microscopy, and confocal laser scanning microscopy. All teeth, except the negative controls, were inoculated with E. faecalis . The teeth were divided into the following groups: group 1, triple antibiotic paste (ciprofloxacin:metronidazole:minocycline) at concentrations of 10, 1, and 0.1 mg/mL; group 2, double antibiotic paste (ciprofloxacin:metronidazole) at concentrations of 10, 1, and 0.1 mg/mL; group 3: Ultracal XS calcium hydroxide (Ultradent, St Louis, MO); and controls, negative and positive controls. Current regenerative protocols recommended by the American Association of Endodontists were followed. S2 sampling was performed after 4 weeks and tested for bacterial presence via culturing, scanning electron microscopy, and confocal laser scanning microscopic analysis. Results The data showed that calcium hydroxide and the current recommended antibiotic concentrations are not capable of completely eliminating bacteria from simulated necrotic immature permanent teeth. Conclusions Overall, this study focuses on the need to re-evaluate the balance between stem cell toxicity and bacterial elimination in order to determine the appropriate concentrations and medicaments for successful regenerative endodontic procedures. |
| Databáze: | OpenAIRE |
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