Anisotropic forces from spatially constrained focal adhesions mediate contact guidance directed cell migration
| Autor: | Patrick W. Alford, Rachel M. Edwards, Paolo P. Provenzano, Arja Ray, Oscar Lee, Deok Ho Kim, Zaw Win |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Science Traction (engineering) General Physics and Astronomy 02 engineering and technology Cell Communication Time-Lapse Imaging General Biochemistry Genetics and Molecular Biology Contact guidance Article Focal adhesion 03 medical and health sciences Mice Optics Collagen fibres Cell Movement Cell Line Tumor Neoplasms Cell Adhesion Animals Humans Anisotropy Cytoskeleton Actin Focal Adhesions Multidisciplinary Microscopy Confocal business.industry Cell migration General Chemistry 021001 nanoscience & nanotechnology Actins Actin Cytoskeleton 030104 developmental biology Biophysics 0210 nano-technology business |
| Zdroj: | Nature Communications, Vol 8, Iss 1, Pp 1-17 (2017) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Directed migration by contact guidance is a poorly understood yet vital phenomenon, particularly for carcinoma cell invasion on aligned collagen fibres. We demonstrate that for single cells, aligned architectures providing contact guidance cues induce constrained focal adhesion maturation and associated F-actin alignment, consequently orchestrating anisotropic traction stresses that drive cell orientation and directional migration. Consistent with this understanding, relaxing spatial constraints to adhesion maturation either through reduction in substrate alignment density or reduction in adhesion size diminishes the contact guidance response. While such interactions allow single mesenchymal-like cells to spontaneously ‘sense' and follow topographic alignment, intercellular interactions within epithelial clusters temper anisotropic cell–substratum forces, resulting in substantially lower directional response. Overall, these results point to the control of contact guidance by a balance of cell–substratum and cell–cell interactions, modulated by cell phenotype-specific cytoskeletal arrangements. Thus, our findings elucidate how phenotypically diverse cells perceive ECM alignment at the molecular level. Contact guidance on aligned substrates leads to directed cell migration through a poorly defined mechanism. Here the authors show that alignment of adhesion structures and F-actin generates anisotropic traction stress to drive directional migration, and cell-cell contact reduces force orientation and directional response. |
| Databáze: | OpenAIRE |
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