Ablation of BMP signaling hampers the blastema formation in Poecilia latipinna by dysregulating the extracellular matrix remodeling and cell cycle turnover
| Autor: | Isha Ranadive, Suresh Balakrishnan, Sonam Patel, Isha Desai, Shailja Rajaram |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0106 biological sciences 0301 basic medicine animal structures DNA Fragmentation Biology Bone morphogenetic protein 010603 evolutionary biology 01 natural sciences Extracellular matrix 03 medical and health sciences Animals Regeneration Protein kinase B PI3K/AKT/mTOR pathway Poecilia Regeneration (biology) Cell Cycle Cell migration Cell cycle Extracellular Matrix Cell biology Pyrimidines 030104 developmental biology Bone Morphogenetic Proteins Animal Fins Pyrazoles Female Animal Science and Zoology Blastema |
| Zdroj: | Zoology. 133:17-26 |
| ISSN: | 0944-2006 |
| Popis: | Bone morphogenetic proteins play a pivotal role in the epimorphic regeneration in vertebrates. Blastema formation is central to the epimorphic regeneration and crucially determines its fate. Despite an elaborate understanding of importance of Bone morphogenetic protein signaling in regeneration, its specific role during the blastema formation remains to be addressed. Regulatory role of BMP signaling during blastema formation was investigated using LDN193189, a potent inhibitor of BMP receptors. The study involved morphological observation, in vivo proliferation assay by incorporation of BrdU, comet assay, qRT-PCR and western blot. Blastemal outgrowth was seen reduced due to LDN193189 treatment, typified by dimensional differences, reduced number of proliferating cells and decreased levels of PCNA. Additionally, proapoptotic markers were found to be upregulated signifying a skewed cellular turnover. Further, the cell migration was seen obstructed and ECM remodeling was disturbed as well. These findings were marked by differential transcript as well as protein expressions of the key signaling and regulatory components, their altered enzymatic activities and other microscopic as well as molecular characterizations. Our results signify, for the first time, that BMP signaling manifests its effect on blastema formation by controlling the pivotal cellular processes possibly via PI3K/AKT. Our results indicate the pleiotropic role of BMPs specifically during blastema formation in regulating cell migration, cell proliferation and apoptosis, and lead to the generation of a molecular regulatory map of determinative molecules. |
| Databáze: | OpenAIRE |
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