Molecular-Recognition-Based DNA Nanodevices for Enhancing the Direct Visualization and Quantification of Single Vesicles of Tumor Exosomes in Plasma Microsamples
| Autor: | Huizhen Wang, See Lok Ho, Hung-Wing Li, Dinggeng He, Xiaoxiao He, Hei Nga Chan, Luo Hai, Kemin Wang |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Aptamer macromolecular substances Biosensing Techniques 010402 general chemistry Exosomes 01 natural sciences Fluorescence Analytical Chemistry chemistry.chemical_compound Molecular recognition Cell Line Tumor Neoplasms Animals Humans Nanodevice Mice Inbred BALB C Total internal reflection fluorescence microscope Chemistry Vesicle fungi 010401 analytical chemistry Optical Imaging food and beverages Receptor Protein-Tyrosine Kinases Equipment Design Aptamers Nucleotide Microvesicles 0104 chemical sciences Cell biology Nanostructures carbohydrates (lipids) enzymes and coenzymes (carbohydrates) DNA |
| Zdroj: | Analytical chemistry. 91(4) |
| ISSN: | 1520-6882 |
| Popis: | Tumor exosomes (Exo) are presumed to expedite both the growth and metastasis of tumors by actively participating in nearly all aspects of cancer development. Tumor-derived Exos are thus proposed as a resource for diagnostic biomarkers in bodily fluids. However, most Exo assays require large samples and are time-consuming, complicated, and costly, and thus unsuited for practical applications. Herein, we show an ultrasensitive assay that can directly visualize and quantify tumor Exos in plasma microsamples (1 μL) at the single-vesicle level. The assay uses the specific binding of activatable aptamer probes (AAP) to target Exos captured by Exo-specific antibodies on the surface of a flow cell to produce activated fluorescence. Furthermore, the bound AAP triggers in situ assembly of a DNA nanodevice with enhanced fluorescence that improves the Exo-detection sensitivity. By identifying tyrosine-protein-kinase-like 7 (PTK7), a total-internal-reflection-fluorescence (TIRF) assay for PTK7-Exo distinguishes target tumors from control subjects. This assay is also informative in monitoring tumor progression and early responses to therapy. The developed assay can be readily adapted for diagnosis and monitoring of other disease-associated Exo biomarkers. |
| Databáze: | OpenAIRE |
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