Toolkit for Visualization of the Cellular Structure and Organelles in Aspergillus niger
| Autor: | Tom Schonewille, Emiel B J Ten Buren, Dorett I Odoni, Vincen Wu, Johanna M Verest, Mark W. J. van Passel, Teresita Bello Gonzalez, Ying Deng, Ruben G. A. van Heck, Shreyans Chordia, JingJing Hu, Leo H. de Graaff, Michiel A P Karrenbelt, Laura van der Straat, Marit Lingemann |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Green Fluorescent Proteins
Biomedical Engineering Biochemistry Genetics and Molecular Biology (miscellaneous) Microbiology Single-cell analysis Chromoprotein Microbiologie Secretion Systems and Synthetic Biology hyphae Cytoskeleton gene Gene Actin VLAG Organelles Systeem en Synthetische Biologie biology business.industry Aspergillus niger fungi General Medicine biology.organism_classification Phenotype Actins Cellular Structures Biotechnology Cell biology Single-Cell Analysis business Biomarkers |
| Zdroj: | ACS synthetic biology 3 (2014) 12 ACS synthetic biology, 3(12), 995-998 |
| ISSN: | 2161-5063 |
| Popis: | Aspergillus niger is a filamentous fungus that is extensively used in industrial fermentations for protein expression and the production of organic acids. Inherent biosynthetic capabilities, such as the capacity to secrete these biomolecules in high amounts, make A. niger an attractive production host. Although A. niger is renowned for this ability, the knowledge of the molecular components that underlie its production capacity, intercellular trafficking processes and secretion mechanisms is far from complete. Here, we introduce a standardized set of tools, consisting of an N-terminal GFP-actin fusion and codon optimized eforRed chromoprotein. Expression of the GFP-actin construct facilitates visualization of the actin filaments of the cytoskeleton, whereas expression of the chromoprotein construct results in a clearly distinguishable red phenotype. These experimentally validated constructs constitute the first set of standardized A. niger biomarkers, which can be used to study morphology, intercellular trafficking, and secretion phenomena. |
| Databáze: | OpenAIRE |
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